Compound Phyllanthus urinaria L Inhibits HBV-Related HCC through HBx-SHH Pathway Axis Inactivation

Figure 3

CP could inhibit migration and clone formation of HepG2-HBx cells. Migration (a), clone formation (b), and the clone numbers ≥50 cells (c) were measured when HepG2-HBx cells were treated with CP (70μg/ml; 35 μg/ml, respectively) and cyclopamine (70μM) for 48 h. The pictures were captured by the Fluorescence Inversion microscope system (×10) or Single Lens Reflex. < 0.001, compared with the group of HepG2-NC; ^##P < 0.01 and ^###P < 0.001, compared with the group of HepG2-HBx.

(a)

(b)

(c)