Antitrypanosomal Effects of Zanthoxylum zanthoxyloides (Lam.) Zepern. & Timler Extracts on African Trypanosomes

Table 4

Effect of Kupchan fractions on cell viability of T. brucei and human cell lines.

FRACTIONS	MEAN   ± SE (μg/ml)			SI
	T. brucei	Jurkat	Chang liver	Jurkat	Chang liver
ZRC	3.41±0.06	NA	NA	NA	NA
ZRFD	5.70 ± 0.03	52.04 ± 0.04	89.93 ± 0.03	9.13	15.78
ZRFH	13.26 ± 0.11	NA	NA	NA	NA
ZRFM	3.89 ± 0.06	68.15 ± 0.05	93.84 ± 0.03	17.52	24.12
ZRWB	4.02 ± 0.06	25.28 ± 0.05	375.58 ± 0.07	6.29	93.43
DA	0.54 ± 0.05	NA	39.72 ± 0.03	NA	73.56
DX	NA	0.27 ± 0.28	145.22 ± 0.02	NA	NA

Mean and standard errors (SE) were calculated from three distinct experiments. SI was calculated as the ratio of in Jurkat or Chang liver cells to the in T. brucei. ZR=Z. zanthoxyloides, root; ZRC=crude extract of ZR; ZRFH= Z. zanthoxyloides hexane fraction; ZRFD= Z. zanthoxyloides Dichloromethane fraction; ZRFM= Z. zanthoxyloides methanol fraction; ZRWB= Z. zanthoxyloides butanol fraction; DA=Diminazene aceturate, (an antitrypanosomal drug); DX=Doxorubicin, (an antileukaemia drug); SI= selectivity index; NA=Not applicable (only ZRFD, ZRFM, and ZRWB were considered for further analysis due to their promising values against T. brucei).