A 4-Week Repeated Oral Dose Toxicity Study of Ssanghwa-Tang in Crl:CD Sprague Dawley Rats
Table 4
Hematological parameters of rats treated with SHT for 4 weeks.
Dose (mg/kg/day)
0
1000
2000
5000
Male
WBC (103/μL)
9.63 ± 1.77
7.83 ± 1.14
8.94 ± 1.13
9.56 ± 2.18
RBC (106/μL)
8.09 ± 0.26
7.88 ± 0.22
8.15 ± 0.20
7.82 ± 0.26
HGB (g/dL)
15.7 ± 0.47
15.5 ± 0.43
15.8 ± 0.64
15.3 ± 0.29
HCT (%)
47.9 ± 1.87
47.1 ± 1.34
48.5 ± 1.98
46.6 ± 0.66
MCV (fL)
59.3 ± 0.90
59.8 ± 1.66
59.5 ± 1.81
59.6 ± 1.50
MCH (pg)
19.4 ± 0.26
19.6 ± 0.53
19.4 ± 0.52
19.5 ± 0.67
MCHC (g/dL)
32.7 ± 0.44
32.8 ± 0.08
32.6 ± 0.53
32.8 ± 0.46
RET% (%)
2.52 ± 0.35
2.53 ± 0.19
2.37 ± 0.42
3.38 ± 0.95
PLT (103/μL)
1013.4 ± 53.30
1047.4 ± 88.88
908.0 ± 58.12
1027.8 ± 96.13
PT (sec)
14.8 ± 0.54
14.5 ± 0.45
15.2 ± 0.76
14.6 ± 0.61
APTT (sec)
16.1 ± 0.23
16.0 ± 0.92
16.3 ± 0.94
15.4 ± 0.47
Neutrophils (%)
13.3 ± 3.41
13.1 ± 3.55
12.2 ± 2.94
12.1 ± 4.87
Lymphocytes (%)
81.9 ± 4.63
82.4 ± 4.29
82.8 ± 3.70
82.9 ± 5.71
Eosinophils (%)
1.0 ± 0.23
1.0 ± 0.19
1.2 ± 0.50
0.9 ± 0.39
Monocytes (%)
2.3 ± 1.11
1.9 ± 0.41
2.2 ± 0.59
2.2 ± 0.70
Basophils (%)
0.3 ± 0.10
0.5 ± 0.29
0.3 ± 0.13
0.3 ± 0.05
LUC (%)
1.0 ± 0.18
1.2 ± 0.55
1.2 ± 0.19
1.6 ± 0.67
Female
WBC (103/μL)
6.77 ± 1.121
7.00 ± 1.406
5.69 ± 1.119
6.46 ± 1.433
RBC (106/μL)
8.16 ± 0.498
8.17 ± 0.460
8.22 ± 0.335
7.62 ± 0.370
HGB (g/dL)
15.8 ± 0.52
15.9 ± 0.67
15.9 ± 0.58
14.8 ± 0.71
HCT (%)
48.2 ± 2.24
48.0 ± 1.59
48.5 ± 1.46
45.6 ± 2.02
MCV (fL)
59.1 ± 1.42
58.8 ± 1.75
59.1 ± 0.83
59.9 ± 1.89
MCH (pg)
19.5 ± 0.72
19.5 ± 0.61
19.4 ± 0.45
19.5 ± 0.76
MCHC (g/dL)
32.9 ± 0.96
33.2 ± 0.51
32.8 ± 0.67
32.5 ± 0.58
RET% (%)
2.10 ± 0.20
2.50 ± 0.85
2.71 ± 0.78
± 1.40
PLT (103/μL)
1006.6 ± 125.33
1106.4 ± 102.83
991.2 ± 160.96
1012.2 ± 117.65
PT (sec)
14.7 ± 0.54
15.2 ± 0.41
15.1 ± 0.54
14.9 ± 0.59
APTT (sec)
13.9 ± 0.93
13.3 ± 1.83
13.6 ± 1.10
13.7 ± 1.58
Neutrophils (%)
12.3 ± 3.25
11.5 ± 3.9
10.8 ± 4.2
14.8 ± 6.2
Lymphocytes (%)
83.3 ± 2.16
84.2 ± 3.9
84.4 ± 4.1
81.2 ± 6.5
Eosinophils (%)
1.1 ± 0.54
1.0 ± 0.1
1.1 ± 0.5
0.9 ± 0.3
Monocytes (%)
1.6 ± 0.48
1.8 ± 0.3
1.8 ± 0.4
1.5 ± 0.3
Basophils (%)
0.5 ± 0.15
0.4 ± 0.1
0.4 ± 0.1
0.4 ± 0.1
LUC (%)
1.3 ± 0.43
1.1 ± 0.4
1.4 ± 0.4
1.2 ± 0.3
Results are presented as mean ± SD. Means Dunn's rank sum test significant at the 0.05 level. WBC, total leukocyte count; RBC, total red blood cell; HGB, hemoglobin; HCT, hematocrit; MCV, mean corpuscular volume; MCH, mean corpuscular hemoglobin; MCHC, mean corpuscular hemoglobin concentration; RET%; relative reticulocyte, PLT; platelet count, PT; prothrombin time; APTT; activated partial thromboplastin time; LUC, Large unstained cells.
