Protective Effects of Evodiamine against LPS-Induced Acute Kidney Injury through Regulation of ROS-NF-κB-Mediated Inflammation

Figure 5

Role of ROS in EVO-induced protective effect against LPS-induced cytotoxicity in NRK-52E cells. NRK-52E cells were treated with 1 μg/ml LPS in the presence or absence of 10 and 20 μM EVO for 24h. Relative mRNA (a) and protein (b) expression of P65 NF-κB was measured. NRK-52E cells were transfected with pCMV vector or pCMV-P65 and exposed to 1 μg/ml LPS with or without 20 μM EVO for 24h. Relative mRNA expression of TNFα (c) and IL-1β (d) was measured. Cell viability was detected by MTT assay (e). #p<0.05 vs control group. ##p<0.05 vs LPS group. ###p<0.05 vs LPS+EVO group.

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