A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Table 2

Calibration curves, linear ranges, correlation coefficients, and LLOQ of gastrodin, -isobutylmalic acid, gymnoside I, and militarine in rat plasma (n=3).

Analytes	Linear regression equation		Linear range (ng /mL)	LLOQ (ng /mL)
militarine	Y=0.0461X - 0.0924	0.9997	1.02 - 97.92	1.02
gastrodin	Y=0.4226X + 0.0112	0.9996	2.96 - 1136.64	2.96
α-isobutylmalic acid	Y=0.2270X + 0.0174	0.9994	1.64 - 629.76	1.64
gymnoside I	Y=0.0436X - 0.0093	0.9996	0.3 - 57.6	0.3