A Validated HPLC-MS/MS Method for Simultaneous Determination of Militarine and Its Three Metabolites in Rat Plasma: Application to a Pharmacokinetic Study

Table 5

Stability of the four analytes in rat plasma under various storage conditions (n = 5).

Analytes	Spiked Concentration (ng/mL)	Post-preparation stability			Freeze-thaw stability
		Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)	Calculated concentration (ng/mL)	Precision (RSD, %)	Accuracy (%)
militarine	2.04	1.85 ± 0.14	7.3	90.6	2.09 ± 0.21	9.9	102.6
	32.64	33.47 ± 2.22	6.6	102.5	35.09 ± 1.41	4.0	107.5
	97.92	96.57 ± 5.42	5.6	98.6	95.68 ± 7.57	7.9	97.7
gastrodin	5.92	6.29 ± 0.60	9.6	106.2	6.11 ± 0.59	9.6	103.2
	378.88	357.97 ± 11.85	3.3	94.5	410.63 ± 26.77	6.5	108.4
	1136.64	1080.94 ± 52.32	4.8	95.1	1087.88 ± 31.11	2.9	95.7
α-isobutylmalic acid	3.28	3.03 ± 0.15	4.9	92.4	3.21 ± 0.27	8.5	97.9
	209.92	213.51 ± 6.62	3.1	101.7	213.32 ± 13.89	6.5	101.6
	629.76	647.02 ± 27.63	4.3	102.7	607.66 ± 32.21	5.3	96.5
gymnoside I	0.60	0.62 ± 0.05	8.6	103.6	0.63 ± 0.05	8.5	104.6
	19.2	18.65 ± 1.34	7.2	97.1	20.14 ± 1.49	7.4	104.9
	57.6	54.29 ± 3.67	6.8	94.3	54.50 ± 1.91	3.5	94.6