Research Article
Protium javanicum Burm. Methanol Extract Attenuates LPS-Induced Inflammatory Activities in Macrophage-Like RAW264.7 Cells
Table 1
High performance liquid chromatography (HPLC) condition conditions to analyze active ingredients.
| Instrument | KNAUER crop. HPLC system | Column | Phenomenex, Gemini 5 μm C18 110A, 250 X 4.60 mm | Detector | UV/VIS detector (370 nm) | Solvent A | 0.1% TFA in H2O | Solvent B | 0.008% TFA in 95% MeCN + 5% H2O | Standard | Dilution with DMSO | Sample treatment | 50mg/ml dilution with DMSO | Injection volume | 20 μl | Flow rate | 1.0 ml/min |
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