Research Article

Protium javanicum Burm. Methanol Extract Attenuates LPS-Induced Inflammatory Activities in Macrophage-Like RAW264.7 Cells

Table 1

High performance liquid chromatography (HPLC) condition conditions to analyze active ingredients.

InstrumentKNAUER crop. HPLC system
ColumnPhenomenex, Gemini 5 μm C18 110A,  
250 X 4.60 mm
DetectorUV/VIS detector (370 nm)
Solvent A0.1% TFA in H2O
Solvent B0.008% TFA in 95% MeCN + 5% H2O
StandardDilution with DMSO
Sample treatment50mg/ml dilution with DMSO
Injection volume20 μl
Flow rate1.0 ml/min