Prophylactic Repetitive Treatment with the Herbal Medicine Kei-kyoh-zoh-soh-oh-shin-bu-toh Attenuates Oxaliplatin-Induced Mechanical Allodynia by Decreasing Spinal Astrocytes
Figure 5
Effect of prophylactic repetitive oral administration of Kei-kyoh-zoh-soh-oh-shin-bu-toh (KSOT) on the expression of NMDA receptor 2B (NR2B) and neuregulin-1 (NRG1) in oxaliplatin-treated mice. Mice were given a single injection of oxaliplatin (OXP, 3 mg/kg, i.p.) or the corresponding vehicle (VH1) on day 0. KSOT or the vehicle (VH2) was orally administered once daily, starting the day after a single injection of OXP or the corresponding vehicle (VH1). The spinal cord for NR2B (a) and the sciatic nerve for NRG1 (b) were isolated on day 10 after OXP injection and the proteins were extracted for Western blotting. The expression level of NR2B or NRG1 in each sample was normalized to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and further normalized to its expression level of the vehicle control (VH1+VH2). (a) A typical example of the expression levels of NR2B or NRG1 and GAPDH. (b) The expression level of NR2B or NRG1, normalized with that of GAPDH. Data are presented as mean ± standard errors of the mean (n = 6 for NR2B; n = 3 for NRG1).