Curcumin Inhibits Joint Contracture through PTEN Demethylation and Targeting PI3K/Akt/mTOR Pathway in Myofibroblasts from Human Joint Capsule

Figure 2

PTEN expression levels in vivo, in vitro, and in the curcumin group. (a, b) Immunohistochemical comparison of the control and the contracture group tissues (magnification, ×200). (c) Polymerase chain reaction revealed that PTEN expression levels in the contracture group were decreased compared with those in the control group, whilst they were elevated in the curcumin group. (d, e) Western blot analysis revealed that PTEN expression in the contracture group was decreased compared with the control group, while it was elevated in the curcumin group. relative to the control group. (f, g) Western blot analysis revealed that PTEN expression in the contracture group was decreased compared with the control group, while it was elevated in the curcumin group. p-PTEN expression in the contracture group was elevated compared with the control group, while it was decreased in the curcumin group. relative to the control group. relative to contracture group. PTEN, phosphatase and tension homolog; p-PTEN, phosphorylation of phosphatase and tension homolog.

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