Curcumin Inhibits Joint Contracture through PTEN Demethylation and Targeting PI3K/Akt/mTOR Pathway in Myofibroblasts from Human Joint Capsule

Table 1

Primers used for reverse transcription-quantitative polymerase chain reaction analysis.

Name	Primer sequence (5′–3′)	Product size (bp)
PTEN	Forward: 5′-TGGATTCGACTTAGACTTGACCT-3′	231
	Reverse: 5′-GGTGGGTTATGGTCTTCAAAAGG-3′
α-SMA	Forward: 5′-ACGAGACCACCTACAACAGCAT-3′	269
	Reverse: 5′-CTCGTCGTACTCCTGCTTGGT-3′
β-Actin	Forward: 5′-AACAGTCCGCCTAGAAGGAC-3′	281
	Reverse: 5′-CGTTGACTACCGTAAAGACC-3′

PTEN: PTEN, phosphatase and tension homolog; α-SMA: α-smooth muscle actin.