Research Article

Cardioprotective Effect of Danhong Injection against Myocardial Infarction in Rats Is Critically Contributed by MicroRNAs

Table 2

Reaction mix for the miRNA PCR array.

Array format: componentVolume (96-well)

2x QuantiTect SYBR Green PCR Master Mix1375 μl
10x miScript Primer assay275 μl
RNase-free water1000 μl
Template cDNA100 μl
Total volume2750 μl

Note. Ensure whether the cDNA was diluted 10 times (25 μl per well).