Cardioprotective Effect of Danhong Injection against Myocardial Infarction in Rats Is Critically Contributed by MicroRNAs

Table 3

Cycling conditions for real-time PCR.

Step	Time	Temperature
PCR initial activation step	15 min	95°C
3-step cycling
Denaturation	15 s	94 C
Annealing	15 s	55 C
Extension	30 s	70 C
Cycle number	40 cycles