Shuang-Huang-Lian Attenuates Airway Hyperresponsiveness and Inflammation in a Shrimp Protein-Induced Murine Asthma Model

Figure 6

Effect of SHL on Th2 cytokines in BALF and their production in MLNs cells. (a) SHL decreased IL-4, IL-5, and IL-13 levels in BALF. (b) SHL decreased IL-4, IL-5, and IL-13 production in MLNs cells. Three days after the last inhalation of SP, mice were sacrificed and the MLNs were isolated. Single cell suspensions were aseptically prepared and seeded in a round bottom 96-well plate (2 × 10⁶ cells/well) and then stimulated with SP (10 μg/mL) at 37°C for 72 h. The levels of IL-4, IL-5, and IL-13 in the culture medium were measured using the ELISA kits. Values were expressed as mean ± SD (n = 8). P < 0.01 versus NS; P < 0.05 and P < 0.01 versus SP.

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