Anti-Inflammatory Effects of Licania macrocarpa Cuatrec Methanol Extract Target Src- and TAK1-Mediated Pathways

Figure 4

Overexpression of Src and TAK1 can be blocked by Lm-ME. (a) HEK293T cells were transfected with HA-Src constructs for 24 h and then divided according to treatment with Lm-ME. By western blotting analysis, total HA and Src and phosphorylated Src and p85 were detected. (b and e) RAW264.7 cells were pretreated with PP2 (an Src family inhibitor) and 5Z-7-oxozeaenol (a TAK1 inhibitor) at different concentrations. After 30 min, LPS was added for 24 h and the supernatant of RAW264.7 cells reacted with Griess reagents. NO production was measured by spectrophotometer. (c and f) The cytotoxicity of PP2 and 5Z-7-oxozeaenol were examined by MTT assay. (d) HEK293T cells were transfected with an HA-TAK1 expression construct for 24 h and also treated with Lm-ME for 24 h. Total HA, TAK1, and MKK4 and phosphorylated TAK1 and MKK4 were determined by western blotting analysis.

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