Oxidative Stress and TGF-β1/Smads Signaling Are Involved in Rosa roxburghii Fruit Extract Alleviating Renal Fibrosis
Figure 5
Cili modulates the mRNA and protein expressions of proteins involved in the TGF-β/Smads pathway in kidneys after UUO. Sixty rats were randomized to six groups: sham operation, UUO, UUO with losartan (UUO+losartan), UUO with moderate Cili dose (3 g/kg/d) (UUO+CL moderate), UUO with high Cili dose (6 g/kg/d) (UUO+CL high), and UUO with resveratrol (UUO+resveratrol). (a) Renal expression of the TGFB1, TGFBR1, TGFBR2, SMAD2, SMAD3, and SMAD7 genes detected by quantitative RT-PCR. GAPDH was used as an internal control, and the relative mRNA level of each gene was normalized to the mRNA levels in the UUO group. (b) Renal expressions of the TGFB1, TGFBR1, TGFBR2, SMAD2, SMAD3, and SMAD7 proteins detected by western blot. N=12/group. The results are expressed as mean ± SD. P<0.01 vs. the sham group;△P<0.05 and △△P<0.01 vs. the UUO group; ◆P<0.05 vs. the losartan group.