Identification of Chinese Herbal Compounds with Potential as JAK3 Inhibitors

Figure 2

Effects of hit Chinses herbal chemicals on JAK3 phosphorylation. IL-2 induced phosphorylated JAK3 protein in HEK-293 cell line was determined by western blot after treatment with phytochemicals at the indicated concentrations for 2 h prior to stimulation with IL-2 for 1 h. GAPDH was used as the internal control for protein normalization.