Protective Effects of Aqueous Extract of Mentha suaveolens against Oxidative Stress-Induced Damages in Human Keratinocyte HaCaT Cells
Induction of HO-1 expression by MSAE in HaCaT keratinocyte cells. After transfection with luciferase reporter plasmid, the cells were pretreated with different concentrations of MSAE for 18h. (a) ARE luciferase activity is presented as fold of untreated controlSD of three independent experiments. To analyze the effect of MSAE or H2O2 on HO-1 expression, HaCaT cells were treated with MSAE for 18 h followed by treatment with 1.6 mM H2O2 for 6 h. (b) Transcriptional levels were detected by RT-PCR. (c) Translational levels were detected by western blot analysis. Data are presented as mean SD of three independent experiments. , compared with untreated cells; ,;, compared with H2O2-treated cells.
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