Antioxidative and Antimelanogenesis Effect of Momordica charantia Methanol Extract
Figure 4
Antimelanogenic effect of Mc-ME in B16F10 cells. (a) B16F10 cells were treated with increasing doses of Mc-ME (0–400 μg/ml) for 24 h, and cell viability was determined by a conventional MTT assay. (b) Total lysates of B16F10 cells treated with either increasing doses of Mc-ME (0–400 μg/ml) or kojic acid (300 μM) for 24 h were incubated with mushroom tyrosinase (100 units/ml) and L-DOPA (1 mg/ml) for 15 min, and tyrosinase activity was determined by measuring the absorbance at 475 nm. (c) B16F10 cells were pretreated with either increasing doses of Mc-ME (0–400 μg/ml) or arbutin (1 mM) for 30 min and then treated with α-MSH (100 nM) for 48 h. The melanin content in the cells was imaged by photography and determined quantitatively by measuring the absorbance at 405 nm. (d) B16F10 cells were pretreated with either increasing doses of Mc-ME (0–400 μg/ml) or arbutin (1 mM) for 30 min and then treated with α-MSH (100 nM) for 48 h. The level of melanin secreted from the cells into culture media was assessed by photography to compare the color of the medium and determined quantitatively by measuring the absorbance at 475 nm. P < 0.05, P < 0.01 compared to control.