Safety Assessment of Methanol Extract of Melastoma malabathricum L. Leaves following the Subacute and Subchronic Oral Consumptions in Rats and Its Cytotoxic Effect against the HT29 Cancer Cell Line

Table 6

Biochemical profiles of liver of Sprague-Dawley rats following the 90-day oral pretreatment with vehicle (Tween 80) or MEMM in the subchronic toxicity study.

Serum liver parameters	Male
	Control	Treatment
	Tween 80	MEMM (mg/kg)
	—	50	500	1000
ALT (U/L)	52.58 ± 4.62	57.65 ± 10.89	55.88 ± 5.78	59.08 ± 7.10
ALP (U/L)	136.80 ± 25.87	151.50 ± 15.88	224.80 ± 37.52	236.50 ± 16.83
AST (U/L)	170.30 ± 16.42	235.60 ± 48.06	224.10 ± 49.70	223.60 ± 45.40
TBil (μmol/L)	1.58 ± 0.31	1.36 ± 0.25	1.43 ± 0.13	1.47 ± 0.18
Creatinine (μmol/L)	61.00 ± 2.12	57.75 ± 2.60	54.75 ± 1.80	58.50 ± 2.50
Urea (mmol/L)	7.15 ± 0.93	7.33 ± 1.07	5.43 ± 0.30	5.75 ± 0.30

Values are expressed as means ± SEM. Significant different as compared to control, p < 0.05. Groups (n = 6): control (treated with Tween 80); treatments (treated with 50 mg/kg MEMM, 250 mg/kg MEMM, and 1000 mg/kg MEMM)