Estrogenic Effect of the Extract of QingYan Formula on Reproductive Tissues in Immature Mice

Figure 5

Effect of QingYan formula extract (QYFE) on expression of estrogen receptor (ER) subtype in the uterus and vagina in immature mice. ERs expressions were assessed by immunohistochemistry. Representative photomicrographs taken at 200‐X magnification in the uterus and 400‐X magnification in the vagina. (a) ERs expression of uterus in immature mice. ERs expressed in the epithelial cells of the endometrium, interstitial cells, and smooth muscle cells in the uterus. (b) ERs expression of vagina in immature mice. ERs were expressed in the vaginal epithelium cells of squamous and smooth muscle cells in vagina. Data are the mean and standard deviation from 10 mice. P values are for the one-way analysis of variance comparing the treatment group with untreated immature mice. Treatment groups are shown: (I) control group; (II) treated with estradiol valerate (EV); (III) treated with QYFE (1 g/kg); (IV) treated with QYFE (2 g/kg); (V) treated with QYFE (4 g/kg). P < 0.001, P < 0.01, and P < 0.05 compared with the control group.

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