FuFangChangTai Decoction Activates Macrophages via Inducing Autophagy

Figure 2

Extraction and identification of autophagy secreted by CT26.WT cells induced by FFCT: (a) morphological changes of CT26.WT cells treated by FFCT by microscope. A. Negative control, B. CT26.WT cells were treated with FFCT (10 mg/mL, 48 h), C. FFCT (20 mg/mL, 48 h), D. FFCT (30 mg/mL, 48 h), E. FFCT (40 mg/mL, 48 h); (b) observation of structural changes of CT26.WT cells treated with FFCT by TEM. A and B. Vesicular granular structure treated by FFCT, C. autophagosome with double-layer membrane structure that encapsulates part of the organelle; (c) expression of autophagy marker protein LC3-II in extract by western blot.

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