Research Article

Pueraria lobata Extract Protects Hydrogen Peroxide-Induced Human Retinal Pigment Epithelial Cells Death and Membrane Permeability

Figure 3

Inhibitory effects of P. lobata extract on ROS generation. Intracellular ROS, detected as H2DCF-DA fluorescence, was measured using FACS. Cells were pretreated with P. lobata extract (10 μg/mL) and its single compounds (1 μM) for 60 min and then cultured for 10 min in the presence of H2O2 (200 μM). Data are expressed as the mean ± SEM. (n = 4). vs. control; vs. H2O2-induced cells.
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