Evidence-Based Complementary and Alternative Medicine / 2019 / Article / Fig 1

Research Article

Targeting Ovarian Cancer Cell Cytotoxic Drug Resistance Phenotype with Xanthium strumarium L. Extract

Figure 1

Impact of the Xanthium strumarium L. extract (XFC) on breast, ovarian, and colorectal cancer cells proliferation. Mid-log phase human cancer cell models derived from breast adenocarcinomas (MCF-7, MDA-MB-231), colorectal adenocarcinomas (HT-29, C2BBe-1), ovarian clear cell carcinoma (ES-2), and ovarian adenocarcinoma (SKOV-3) were seeded in 96-well plates at 103 cells/well density in 200 μL medium and cultured as described in Section 2. Cells were then (a) treated with various concentrations of XFC for 72 hours and then incubated with MTT (0.5 mg/mL) for 4 hours in order to quantify cell proliferation (a representative curve is shown for each cell line). (b) IC50 values were obtained for cell line treatments, which were performed in triplicate.
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