Safflower Yellow B Protects Brain against Cerebral Ischemia Reperfusion Injury through AMPK/NF-kB Pathway
The relationships between AMPK and NF-κB in PC12 cells subjected to I/R. PC12 cells were transfected with siRNAs directed against AMPK (siAMPK) or nontargeting control sinRNA (scrb), continuously incubated with SYB for 24 h, and then subjected to I/R. The proteins in nuclear and cytoplasm were lysed by nuclear and cytoplasmic extraction reagent kits (a). Effects of SYB on the phosphorylation levels of AMPK in PC12 cells after siAMPK translation (b). Effects of SYB on the phosphorylation levels of ACC in PC12 cells after siAMPK translation (c). Effects of SYB on the expression levels of NF-κB p65 in nuclear of PC12 cells after siAMPK translation. siAMPK abolished the effects of SYB on the levels of IL-1 (d), IL-6 (e), and cell viability (f). Data were presented as mean ± SD. ##P<0.01 versus control group; .01 versus I/R group; &&P<0.01 versus SYB treatment group.