Research Article

Effect of Puerarin, Baicalin and Berberine Hydrochloride on the Regulation of IPEC-J2 Cells Infected with Enterotoxigenic Escherichia coli

Figure 7

The expression of proteins related to NF-κB signaling pathway in IPEC-J2 cells. (a) Relative expression of IκBα, total β-actin, cytoplasm P65, cytoplasm β-actin, nucleus P-P65, nucleus P65, and Lamin A/C in IPEC-J2 cells was assayed through western blot. (b–d) Quantitation of relative protein levels of IκBα, cytoplasm P65/nucleus P65, and nucleus P-P65/ P65 in (a), respectively. Control: control cells without any processing; ETEC: treatment with ETEC for 3h; puerarin: pretreatment with puerarin at 200 μg/mL + ETEC; baicalin: pretreatment with baicalin at 1 μg/mL + ETEC; berberine hydrochloride: pretreatment with berberine hydrochloride at 100 μg/mL + ETEC. Data are mean ± SEM, n = 3 per treatment (P < 0.05; P < 0.01).
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