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Evidence-Based Complementary and Alternative Medicine
Volume 2019, Article ID 7481341, 8 pages
https://doi.org/10.1155/2019/7481341
Research Article

Effect of Terminalia catappa Linn. on Biofilms of Candida albicans and Candida glabrata and on Changes in Color and Roughness of Acrylic Resin

1Department of Dentistry, Post-Graduate Program in Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil
2Department of Dentistry, CEUMA University, Sao Luis, Maranhao, Brazil
3Laboratory of Applied Microbiology, Post-Graduate Program in Microbial Biology, CEUMA University, Sao Luis, Maranhao, Brazil
4Graduate Program in Dentistry, Federal University of Maranhao. UFMA, Sao Luis, Maranhao, Brazil

Correspondence should be addressed to Letícia Machado Gonçalves; moc.liamg@gm.stel

Received 28 April 2019; Revised 16 June 2019; Accepted 26 June 2019; Published 7 July 2019

Academic Editor: Raffaele Pezzani

Copyright © 2019 Letícia Machado Gonçalves et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study aimed to investigate the effect of the n-butanol fraction of Terminalia catappa Linn., (FBuTC) on biofilm of Candida albicans and Candida glabrata, as well as changes in color and roughness of polymethyl methacrylate resin (PMMA). The susceptibility of C. albicans and C. glabrata to FBuTC was evaluated by means of the Minimum Inhibitory and Minimum Fungicidal Concentration (MIC and MFC). PMMA acrylic resin discs (N= 108) were fabricated. For the susceptibility tests, biofilms of C. albicans and C. glabrata were developed on discs for 48 h and immersed in phosphate-saline buffer solution (PBS), 1% sodium hypochlorite (SH 1%), or FBuTC at MIC, 5xMIC, or 10xMIC. For the color and roughness change tests, the discs were immersed in distilled water, SH 1%, or FBuTC in the concentrations of 0.25 mg/mL, 2.5 mg/mL, or 25.0 mg/mL. After 28 days of incubation, color change was evaluated by spectrophotometry and roughness, by using a profilometer. The biofilms were investigated by one-way ANOVA and, the color and roughness changes (two-way ANOVA and the Tukey test; α=0.05). For both MIC and MFC the value of 0.25 mg/mL of FBuTC was observed for the planktonic cells of C. albicans and C. glabrata. Exposure to FBuTC at 10xMIC had a significant effect on the biofilm of C. albicans, showing a reduction in cell counts when compared with PBS, (p=0.001). For the biofilm of C. glabrata, the MIC was sufficient for significantly reducing the cell count (p<0.001). No important changes in color and roughness of the acrylic resin were observed, even after 28 days, irrespective of the concentration of FBuTC used (p >0.05). It could be concluded that the immersion of acrylic resin for dental prosthesis in FBuTC was effective in reducing the biofilms of C. albicans and C. glabrata without evidence of change in roughness and color of this substrate.