Enhancement of Endometrial Receptivity by Cnidium officinale through Expressing LIF and Integrins

Figure 4

Effect of antagonist for LIFR and neutralization for integrins αV, β3, and β5 on the adhesion of JAr cells to CoM-stimulated Ishikawa cells. (a) Ishikawa cells were treated with or without LIF antagonist (hLA) (50 ng/mL) for 1 h and then CoM (50 μg/ml) was added to hLA-pretreated Ishikawa cells for 48 h. (b) Ishikawa cells (1.5 × 10⁶ cells) were cultured in 6-well plates and treated with or without CoM (50 μg/ml) for 48 h, and the cells were then incubated in the presence of integrin αV, β3, β5, and IgG antibodies for 2 h. CMFDA-labeled JAr cells were added onto Ishikawa cell monolayer. The attached cells were fixed and pictured using fluorescent microscopy. Data were calculated as mean ± SEM of three independent experiments and expressed as fold of controls. compared to each group (magnification ×50; scale bar = 5 μm).

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