Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 2

Analytical conditions of KCHO-1 using HPLC.

Mobile phase	Time (min)	Water (%)	Acetonitrile (%)
	0	90	10
	30	90	10
	70	13	87
	72	13	87
	73	90	10
	85	90	10
Flow rate	1.0 mL/min
Injection volume	20 μL
Column	CAPCELLPAK 5 C18, 250 2.6 mm, 5 μm
Column oven temperature	40°C
Detector wavelength (PDA)	254 (Glycyrrhizic acid), 280 (Salvianolic acid B), 420 (Curcumin) nm
Run time	85 min