Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 8

Homogeneity of dosing formulation.

(a) Curcumin

Concentration of dosing formulation (μg/mL)		Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
		No.1	No.2	No.3
39.5	Upper	46.67	47.97	46.87	46.47	1.17	2.52	117.65
	Middle	46.66	46.08	46.08
	Lower	43.99	47.82	46.06
158.0	Upper	177.77	182.59	187.16	183.13	3.17	1.73	115.91
	Middle	186.58	182.00	184.79
	Lower	183.97	179.00	184.33

(b) Glycyrrhizic Acid

Concentration of dosing formulation (μg/mL)		Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
		No.1	No.2	No.3
305.0	Upper	252.52	260.77	261.73	248.68	8.32	3.35	81.53
	Middle	238.85	248.52	245.37
	Lower	243.74	247.37	239.27
1220.0	Upper	975.53	947.51	962.39	963.45	20.06	2.08	78.97
	Middle	938.64	955.67	954.80
	Lower	951.83	980.93	1003.77

(c) Salvianolic Acid B

Concentration of dosing formulation (μg/mL)		Measured concentration (μg/mL)			Mean (μg/mL)	SD	CV (%)	Accuracy (%)
		No.1	No.2	No.3
985.0	Upper	966.20	1001.76	988.87	960.32	22.91	2.39	97.49
	Middle	934.32	958.07	945.25
	Lower	938.01	964.54	945.84
3940.0	Upper	3739.49	3721.20	3813.87	3749.74	52.53	1.40	95.17
	Middle	3759.39	3704.46	3731.31
	Lower	3694.78	3728.22	3854.97