Analytical Method Validation of Gamijakyakgamchobuja-Tang (KCHO-1, Mecasin) Preparation

Table 9

Stability of the dosing formulations for 4 hours at room temperature.

(a) Curcumin

Time (hr)	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	39.5	46.52	45.79	46.33	46.21	0.38	0.82	116.99	-
	158.0	186.52	180.49	184.37	183.79	3.06	1.66	116.32	-
4	39.5	46.37	45.93	44.89	45.73	0.76	1.66	115.77	-1.04
	158.0	178.89	181.59	188.74	183.07	5.09	2.78	115.87	-0.39

(b) Glycyrrhizic Acid

Time (hr)	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	305.0	234.75	240.17	244.67	239.86	4.97	2.07	78.64	-
	1220.0	961.40	959.78	971.12	964.10	6.13	0.64	79.02	-
4	305.0	254.16	240.70	233.31	242.72	10.57	4.36	79.58	1.19
	1220.0	947.84	973.20	949.28	956.77	14.24	1.49	78.42	-0.76

(c) Salvianolic Acid B

Time (hr)	Concentration of the dosing formulation (μg/mL)	Measured concentration			Mean (μg/mL)	SD	CV (%)	Accuracy (%)	Variation (%)
		(μg/mL)
		No.1	No.2	No.3
0	985.0	932.67	944.18	948.37	941.74	8.13	0.86	95.61	-
	3940.0	3762.21	3675.21	3755.96	3731.13	48.53	1.30	94.70	-
4	985.0	964.42	938.51	913.89	938.94	25.27	2.69	95.32	-0.30
	3940.0	3680.07	3779.39	3791.90	3750.45	61.27	1.63	95.19	0.52