Evidence-Based Complementary and Alternative Medicine

Evidence-Based Complementary and Alternative Medicine / 2019 / Article
Special Issue

Natural Products and/or Isolated Compounds on Wound Healing

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Review Article | Open Access

Volume 2019 |Article ID 7957860 | https://doi.org/10.1155/2019/7957860

Richard Komakech, Motlalepula Gilbert Matsabisa, Youngmin Kang, "The Wound Healing Potential of Aspilia africana (Pers.) C. D. Adams (Asteraceae)", Evidence-Based Complementary and Alternative Medicine, vol. 2019, Article ID 7957860, 12 pages, 2019. https://doi.org/10.1155/2019/7957860

The Wound Healing Potential of Aspilia africana (Pers.) C. D. Adams (Asteraceae)

Guest Editor: Abidemi J. Akindele
Received20 Aug 2018
Accepted23 Dec 2018
Published21 Jan 2019

Abstract

Wounds remain one of the major causes of death worldwide. Over the years medicinal plants and natural compounds have played an integral role in wound treatment. Aspilia africana (Pers.) C. D. Adams which is classified among substances with low toxicity has been used for generations in African traditional medicine to treat wounds, including stopping bleeding even from severed arteries. This review examined the potential of the extracts and phytochemicals from A. africana, a common herbaceous flowering plant which is native to Africa in wound healing. In vitro and in vivo studies have provided strong pharmacological evidences for wound healing effects of A. africana-derived extracts and phytochemicals. Singly or in synergy, the different bioactive phytochemicals including alkaloids, saponins, tannins, flavonoids, phenols, terpenoids, β-caryophyllene, germacrene D, α-pinene, carene, phytol, and linolenic acid in A. africana have been observed to exhibit a very strong anti-inflammatory, antimicrobial, and antioxidant activities which are important processes in wound healing. Indeed, A. africana wound healing ability is furthermore due to the fact that it can effectively reduce wound bleeding, hasten wound contraction, increase the concentration of basic fibroblast growth factor (BFGF) and platelet derived growth factor, and stimulate the haematological parameters, including white and red blood cells, all of which are vital components for the wound healing process. Therefore, these facts may justify why A. africana is used to treat wounds in ethnomedicine.

1. Introduction

A wound can be defined as the disruption of living tissue integrity associated with loss of function [1]. The wound healing process is a complex dynamic process which represents an attempt to restore a normal anatomical structure and function [2, 3]. Wounds can be broadly categorized as acute wounds which are caused by external injury to the skin and include surgical wounds, bites, burns, minor cuts and abrasions, and more severe traumatic wounds such as lacerations and those caused by crush or gunshot injuries or chronic etiology wounds which includes vascular, diabetic, and pressure ulcers [1, 4]. In fact, wounds impose significant health, social, and economic burdens to the individuals, the healthcare system, and the community as a whole [5, 6]. Recent statistics showed that approximately 3% of the healthcare budget is spent on treating wound-related complications in developed countries [6]. The aim of treating a wound is to prevent pain discomfort to the patient and promote wound healing which occurs mainly in four phases: hemostasis, inflammation, proliferation, and remodeling [1, 7, 8]. Plants have immense potential that can be explored for the treatment and management of wounds [2, 9]. Indeed, several medicinal plants have been used in traditional medicine for the treatment and management of all kinds of wounds across the globe since time immemorial [3, 10, 11]. Aspilia africana (Pers.) C. D. Adams (Asteraceae), commonly referred to as wild sunflower, is one of the highly valued wound healing plants throughout its distribution range and beyond [1214]. This unique wound healing plant species is commonly referred to as “haemorrhage plant” due to its distinguished ability to stop bleeding, even of severed artery [15, 16]. Apart from its enormous potential in wound healing, A. africana is reported to be vital in the treatment and management of myriad of other diseases and disorders in African traditional medicine, including headache, corneal opacities, stomach disorders, cough, gonorrhea, rheumatic pains, and tuberculosis; the leaf infusion is taken as a tonic for women immediately after delivery [17, 18]. A. africana plant is known to possess great anti-inflammatory, antimalarial, and antimicrobial activities [12, 16]. Several scientific studies have attributed the numerous medicinal properties of A. africana to the abundant bioactive secondary metabolites in it such as alkaloids, saponins, tannins, glycosides, flavonoids, and terpenoids [18, 19].

The use of A. africana in wound treatment and management has been assessed and discussed in a number of peer reviewed journal articles over the years. This review therefore sought to examine the wound healing potential of A. africana both in vitro and in vivo with the goal of finding new drugs for treatment and management of wounds.

2. Methods

In this review, we obtained information from original peer reviewed articles published in scientific journals, with a focus on the botany, distribution, and potential of A. africana for treatment and management of wounds. We critically searched electronic literature databases including but not limited to Google Scholar, PubMed, and Scopus for all available peer reviewed data. The following key search terms were used (“A. africana” OR “Wild sunflower” AND “wounds” OR “wound healing” OR “Phytochemicals”) OR (“Phytochemicals in A. africana” OR “Wild sunflower” AND “wound” OR “wound healing”), OR (“Phytochemicals in A. africana” OR “Wild sunflower” AND “Anti-inflammation” OR “Anti-microbial”), OR (“Plants” OR “Natural products” AND “wound” OR “wound healing”) OR (“A. africana” OR “Wild sunflower” AND “Botany” OR “Distribution”). The data obtained were verified independently for their accuracy and any inconsistencies were settled through discussions between the authors. The final data obtained through discussions among the authors were then summarized, analyzed, and compared, and conclusions were made accordingly.

3. Botany and Distribution of Aspilia africana

The genus Aspilia is a genus of common herbaceous flowering plants which are native to Africa and comprised of approximately 140 species [18, 64]. Morphologically, A. africana is a herb measuring about 1-2 m in height covered with bristles; stem is stiff at the base, with many branches and rough to touch (Figures 1(a) and 1(b)); leaves are rough, opposite, ovate-lanceolate, creased accordion-style covered with trichomes, average 10 cm long and 5 cm wide, and rounded at the base with petioles about 1 cm long with 3 prominent veins (Figure 1(c)); inflorescence consists of capitula which is terminal, solitary, or in lax racemes with hairy stalk of about 7 cm long on average; flowers have numerous showy-yellow florets; fruits are 4-angled achenes (Figure 1(d)) [12, 64, 65].

A. africana is native to Africa occurring in a number of countries throughout the tropical African region on waste land of the savanna and forested zones between altitude of 800 and 1800 m (Figure 2), and its rapid growth characteristics make it a difficult weed in cultivated land and fallows [65].

4. Toxicological Effects of Aspilia africana

Generally, this unique wound healing plant can be classified among agents with low toxicity [66]. In an in vivo study by Okokon et al. [67] using Swiss albino mice, the acute toxicity of the ethanolic extract of the plant showed that doses of 2000 mgkg−1 and above were lethal to the animals and the determined LD50 of the extract was 1414.2 mgkg−1. Further, in vivo study by Oko et al. [68] on Swiss albino mice concluded that oral administration of up to 10,000 mgkg−1 body weight of aqueous and ethanolic extracts of the plant was safe for animal and human use. However, a recent study showed that the aqueous leaf extract of A. africana may be teratogenic to the developing placenta of Wistar rats in a dose-dependent manner; more severe outcomes were observed in female rats that received up to 1250 mg/kg body weight of the aqueous extract [69]. Similarly, other previous studies also showed that intraperitoneal administration of the extracts of A. africana leaf caused significant delay in estrus cycle and in addition did not only distort the histology of ovaries and reduce its weight, but also damaged the uterine tissues and fallopian tubes in Wistar rats [17, 67, 70, 71]. Furthermore, methanolic extracts of A. africana have also been found to significantly decrease the weight of testis, epididymis, seminal vesicle, and prostate gland of experimental male Wistar rats [72]. Therefore, despite the safety associated with A. africana, caution must be taken during its long term oral consumption as it may have adverse effects on reproductive organs.

5. Effects of Leaf Extracts of Aspilia africana on Wound Healing

A. africana is one of the many medicinal plants containing large quantities of bioactive compounds making it such a potent plant in wound sepsis treatment and management and other microbe induced disease conditions [19, 20]. Over the years, several in vitro and in vivo scientific studies have been conducted to validate the wound healing ability of this plant. In an in vivo study by Eweka and Eweka [73]; they examined the effects of aqueous extract of A. africana administered orally for fourteen days on the duodenum of adult Wistar rats exposed to varied concentrations of hydrochloric acid. The histological findings indicated sections of the small intestine of treated rats showed varying degrees of cellular proliferation and epithelia regeneration. This showed that A. africana consumption may have antiulcer effects on duodenal ulcer by its healing effects on the Brunnals gland and epithelia cells of the small intestine of adult Wistar rats. Similarly, earlier study by Nguelefack et al. [74] also showed that the methanolic extract of fresh leaves of A. africana at the dose of 1 g/kg reduced gastric lesion in the pylorus ligated rats by 52%, a further proof of the potential of A. africana in wound healing. In a study by Attama et al., 2011 [75] where they examined the methanol leaf extract of A. africana formulated as gels for its potency on experimentally induced wound in rats, 100% wound closure was observed by the 17th day of treatment in both gel formulations of the plant methanolic extract and the standard gel, an indication of effectiveness of A. africana in wound healing. Similarly, a study by Osunwoke et al. [76] on the wound healing effects of the leaves extract of A. africana on Wistar rats showed that the rate of contraction of the excised wounds in the experimental group on days 6 and 9 was significant (P<0.05) with a mean wound closure of 12.6±1.17 cm compared to those in the control group which was 15.0±1.86 cm. Furthermore, they observed that the concentration of neutrophils and macrophages was intense in the experimental group relative to than the control group in the excised tissue samples. The total wound closure and increased inflammatory response suggests that the aqueous extract of the leaves of A. africana promotes wound healing activity through increased inflammatory response and neovascularization. In another in vivo experimental evaluation by Okoli et al. [12] using Wistar rats, they observed that the methanolic and hexane extracts and methanolic fractions of A. africana significantly (P<0.05) reduced bleeding (clotting time) in the rats and caused varying degrees of inhibition of the growth of microbial organisms known to cause wound infections such as Pseudomonas fluorescens and Staphylococcus aureus. The study showed that the extracts reduced epithelialization period of wounds that were experimentally excised in the rats, hence validating the fact that A. africana possesses constituents capable of accelerating wound healing. At different concentrations, A. africana also showed varied stimulating effects on haematological parameters including white and red blood cells due to the enormous micronutrients found in the plant [77]. Indeed, increased haematological changes especially in the red blood cells count are known to result in increased level of oxygen supply to the wounds resulting in faster wound healing [78]. Additionally, the wound healing ability of A. africana has also heavily been attributed to its anti-inflammatory activity resulting in inhibition of prostaglandins synthesis, decreased vascular permeability, inhibition of neutrophil migration into inflamed tissues, and stimulation of lymphocyte accumulation, thus enhancing tissue repair and healing [12]. Indeed, anti-inflammatory activity is essential for wound healing, since a long duration of the inflammatory phase causes delay in the wound healing process [79]. Additionally, the strong antimicrobial activities of A. africana play a vital role in the ability of this plant to heal wound sepsis [8084]. In fact, a study by Anibijuwon et al. [85] showed that A. africana has strong antimicrobial activities. These findings further showed that the anti-inflammatory and antimicrobial agent play vital roles in wound healing process.

6. The Potential of the Phytochemicals from Aspilia africana in Wound Healing

As discussed above, in vivo studies have provided strong pharmacological evidence for wound healing potential of the extracts obtained from A. africana. The plant is endowed with myriad of classes of bioactive secondary metabolites including alkaloids, saponins, tannins, flavonoids, and phenols (Figure 3) [12, 18, 20, 86, 87] and terpenoids [19, 20]. A. africana also contains a number of other compounds (Table 1) such as sesquiterpenes including β-caryophyllene and germacrene D, and linolenic acid [20]. The presence of these phytochemicals suggests that A. africana might be of medicinal importance and supports the basis for its use in ethnomedicine as a wound healing plant.


S/NoClass of compoundPhytochemical compoundsCompound structureActivities that enhances wound healingReference

aMonoterpenescarene(i) Anti-inflammatory
(ii) Antimicrobial
[1926]

bPhytocannabinoidsCaryophyllene(i) Antimicrobial
(ii) Anti-inflammatory
[19, 2735]

cSesquiterpenesGermacrene D(i) Anti-inflammatory
(ii) Anti-microbial and
(iii) Anti-oxidant
[3644]

dTerpeneα-pinene(i) Anti-microbial
(ii) Anti-inflammatory
(iii) Increases basic fibroblast growth factor (BFGF)
(iv) Increases platelet derived growth factor
[4553]

eAcyclic diterpene alcoholPhytol(i) Induces oxidative stress on microbial organisms
(ii) Reduces interleukin-1β and tumor necrosis factor-α levels
[4, 20, 5460]

fFatty acidLinolenic acid(i) Anti-microbial
(ii) Down regulate inflammatory inducible nitric oxide synthase (iNOS).
[20, 6163]

The high content of alkaloids in A. africana may be one of the major contributing factors to the wound healing activity of this plant [64, 68]. A number of alkaloids have been known to have great wound healing activities [18]. In an in vivo study, topical application of an alkaloid enriched-ointment exhibited higher dermal healing activity of the wounds on rats [45]. Similarly, alkaloids have been observed to promote early phases of wound healing in a dose-dependent manner with the ability to stimulate chemotaxis for fibroblasts in vitro [89]. Alkaloids have also been observed to enhance significant wound healing activity (P<0.05) as evidenced by the increased rate of wound contraction and reduction in the period of epithelialization [90]. Sahib et al. [21] reported that the wound healing potential of Ruta graveolens L. plant may be due to the presence of alkaloids. These findings therefore suggest that the wound healing potential of A. africana may be due to the presence of large quantity of alkaloids.

Flavonoids are antioxidants with free radical scavenging ability and are therefore able to prevent oxidative damage in cells and have great anti-inflammatory activities [22], a basis for wound healing. Furthermore, flavonoids are also known to promote the wound healing process mainly due to their astringent and antimicrobial properties which are responsible for wound contraction and increased rate of epithelialization [23, 24]. Consequently, the wound healing ability of flavonoids has been observed to be even greater than that of silver sulfadiazine [25]. Flavonoids have also been observed to increase collagen synthesis, promote the cross-linking of collagen, shorten the inflammation period, and provide resistance against infections, important factors in enhancing the wound healing process [26]. These findings in part may be the reason behind the use of A. africana in the treatment of wounds, ulcers, and burns in traditional medicine.

Saponins’ antioxidant and haemolytic properties make them one of the most important secondary metabolites in the treatment and management of a number of diseases, including wound healing [28, 29]. Indeed, saponins’ ability to treat wounds and stop bleeding is due to the fact these phytochemicals precipitate and aggregate red blood cells [18]. Saponins are also known to enhance wound healing by causing wound contraction and bringing about high collagen deposition [29, 30]. In fact, saponins are also known to promote angiogenesis during skin wound repair [31]. Therefore, the high quantity of saponins in A. africana could explain why the plant has got such a potent ability to treat wounds in traditional medicine.

The presence of phenols in the plant leaf extract of A. africana is an indication that the extract may have antimicrobial properties [18] which greatly offers a basis for wound healing.

Tannins have been reported as having astringent activities which helps to quicken wound healing and treat inflammations [18]. Owing to its antibacterial activity and NIH3T3 cell proproliferative effect, tannins have been observed to promote wound contraction, improve healing rate, and promote healing of infectious wounds [32]. Specifically, tannins have been observed to reduce colonization of wounds by S. aureus resulting in a hasten wound healing [33]. Therefore, the presence of tannins may be one of the reasons why A. africana is renowned for wound healing in traditional medicine.

Terpenoids isolated from the leaves of A. africana include 3β-O-[α-rhamnopyranosyl-(1→6)-β-glucopyransyl-(1→3)-ursan-12-ene, 3β-Hydroxyolean-12-ene, and 3β-acetoxyolean-12-ene (Figure 4) [27]. Other terpenes present include α-pinene [34], carene, and phytol [19, 20] (Table 1).

Terpenoids are known to promote the wound healing process, mainly due to their astringent and antimicrobial properties, which seem to be responsible for wound contraction and an increased rate of epithelialization [35]. Carene (monoterpene) (Table 1) wound healing ability may be due to its antimicrobial activity in which it can inhibit the growth of S. aureus and P. aeruginosa in wounds [3640]. Carene as an example of monoterpenes exhibited strong anti-inflammatory activity [41]. Therefore, the anti-inflammatory and antimicrobial activities of carene and other monoterpenes contained in A. africana somewhat validate the use of this plant in wound healing.

Alpha-pinene (Table 1) is an organic compound of the terpene class contained in A. africana [34, 42]. This vital compound was found to have potent anti-inflammatory activity [43]. The anti-inflammatory activity is due to its ability to suppress mitogen-activated protein kinases (MAPKs) and the nuclear factor-kappa B (NF-κB) pathway which makes it a vital compound in the treatment of inflammatory diseases [44]. Beside its anti-inflammatory activity, singly or in synergy with other compounds, α-pinene has been observed to have interesting antimicrobial properties [4648]. An in vivo study on Pistacia atlantica resin extract with 46.57%  α-pinene as the main content had a concentration-dependent effect on the healing of burn wounds after 14 days of treatment by increasing the concentration of basic fibroblast growth factor (BFGF), platelet derived growth factor, and improving angiogenesis [49]. Indeed, increased concentration of basic fibroblast growth factor is known to greatly enhance wound healing [49, 50]. Therefore, the antimicrobial, anti-inflammatory, and ability to increase BFGF level may explain why A. africana with α-pinene as one of the major compounds has been used in wound healing for generations.

Phytol (Table 1) is an acyclic diterpene alcohol with a percentage abundance of about 13% in the chloroform extract of A. africana [20]. This phytochemical has been shown to have wound healing activity. In an in vivo study, topical application of Stachytarpheta jamaicensis plant extract cream containing phytol on diabetic excision wound significantly improved (P<0.05) the percentage of wound contraction (88%) when compared to untreated diabetic rats in a period of 20 days [51]. It is important to note that wound healing can be greatly delayed due to infection by microorganisms [4]. Pseudomonas aeruginosa is one of the most common bacteria isolated from chronic wounds and can express virulence factors on the surface proteins affecting wound healing [52]. Phytol is known to exert antibacterial property on P. aeruginosa via inducing oxidative stress [53]. Indeed, this compound is known to have high antimicrobial activity, high stability, and low toxicity [54]. In addition to the antimicrobial potential, phytol is also known to be one of the compounds with highly potent anti-inflammatory property [55, 56]. An in vivo study showed that phytol attenuated the inflammatory response by inhibiting neutrophil migration that is partly caused by reduction in interleukin-1β and tumor necrosis factor-α levels and oxidative stress [57]. The presence of phytol in A. africana therefore may explain why this plant has great antimicrobial and anti-inflammatory activities and hence its potent wound healing ability.

Caryophyllene (Table 1) is a natural bicyclic sesquiterpene that is a constituent of many essential oils belonging to a class of phytocannabinoids, one of the many compounds found in the extract of A. africana [19]. This compound has been shown to have potent antimicrobial property [58, 59]. Indeed, β-caryophyllene has demonstrated selective antibacterial activity against S. aureus (minimum inhibitory concentration (MIC) 3±1.0 μM) and more pronounced antifungal activity [60]. Similarly, β-caryophyllene presented rapid bacterial killing for S. aureus (MIC <1.0 mg/Ml) in 4 h [61]; S. aureus is one of the main microbial organisms that enhances wound sepsis [62]. β-caryophyllene has also been shown to exhibit great anti-inflammatory activity [63, 91, 92]. In a study by Dahham et al. [93], it was observed that β-caryophyllene elicited significant (P<0.01) reduction in paw volumes and low intensity of fluorescent signal in experimental animals when compared with negative control. Furthermore, the result indicated that the compound has a low toxicity, with high ability of skin penetration, greatly enhancing anti-inflammatory and analgesic activities making it useful for prevention and management of inflammation-related diseases, including wounds. Therefore, the antimicrobial and anti-inflammatory activities exhibited by β-caryophyllene contained in the extracts of A. africana could explain why this plant is so effective in wound healing.

Germacrene D (Table 1) is a volatile organic hydrocarbon compound belonging to the class sesquiterpenes contained in A. africana plant [27, 94, 95]. The compound possesses potent antimicrobial, anti-inflammatory, and antioxidant potentials activities [9699]. Indeed germacrene D showed broad spectrum antibacterial activity against important human pathogenic Gram-positive and Gram-negative bacteria including S. aureus [100102]. Therefore, the antimicrobial and anti-inflammatory activities exhibited by germacrene D contained in the extracts of A. africana could explain why this plant is so effective in wound treatment and management. However, more studies on isolated germacrene D needs to be conducted to validate further its wound healing potential.

Linolenic acid (Table 1) has been reported to have very strong antimicrobial activity against a number of microbes including those known to infect wounds and delay its healing such as S. aureus [103]. In addition, it is also an important anti-inflammatory agent [104]. Linolenic acid has been observed to down regulate inflammatory inducible nitric oxide synthase (iNOS), cyclooxygenase-2, and tumor necrosis factor-alpha gene expressions through the blocking of nuclear factor-kappaB and mitogen-activated protein kinases activation in lipopolysaccharide-stimulated murine macrophages cell line (RAW 264.7 cells), which may be the mechanistic basis for the anti-inflammatory effect of linolenic acid [105]. The presence of linolenic acid in A. africana therefore may explain why this plant has great antimicrobial and anti-inflammatory activities and hence its potent wound healing ability.

Through synergistic interactions of the different phytochemicals in A. africana, the plant has exhibited very strong antimicrobial, anti-inflammatory, and antioxidant activities which are vital components of the wound healing processes.

7. Conclusion

Throughout the world, wounds impose significant health burdens on millions of people. Consequently, all possible measures have to be taken to tackle it. Natural products have been used over the years for treatment and management of wounds. A. africana is one of the plants with immense attributes to enhance wound healing. The synergistic effects of the major phytochemicals in A. africana including alkaloids, saponins, tannins, flavonoids, β-caryophyllene, germacrene D, α-pinene, carene, phytol, and linolenic acid confer potent anti-inflammatory, antimicrobial, and antioxidant activities on the plant. This probably explains why this plant has such a potent wound healing ability. However, due to the reported adverse effects on the reproductive organs of the experimental animal models when administered orally, we recommend that future clinical studies focus on its topical application for wounds. Furthermore, although several studies have been carried out regarding chemical screening in A. africana, our review did not find any study on major nonvolatile chemical isolation and structure determination except for a limited study on terpenoids. Therefore, further studies on A. africana need to be done in this regard. Future studies also need to focus on the wound healing potential of the individual isolated compounds in A. africana. Furthermore, more preclinical and subsequently clinical studies need to be done to validate and understand the mechanism(s) of action of these phytochemicals in A. africana either in isolation or in combination for possible future wound healing drug development.

Disclosure

Richard Komakech is first author.

Conflicts of Interest

The authors declare that they have no conflicts of interest.

Authors’ Contributions

Richard Komakech carried out the data search and was the major contributor in writing the manuscript. Motlalepula Gilbert Matsabisa and Youngmin Kang technically designed and helped in writing the manuscript. All the authors read and approved the final manuscript.

Acknowledgments

In a special way, we would like to thank Mr. Gang Roggers, a research officer at the National Agricultural Research Organisation (NARO), Ministry of Agriculture and Fisheries in Uganda, for the A. africana pictures that he provided from which the illustrations were made that greatly improved this manuscript. This work was supported under the framework of International Cooperation Program (Korea-South Africa Cooperative Research Project for Excavation of Candidate Resources of Complementary & Alternative Medicine) managed by the National Research Foundation of Korea (Grant 2017093655, KIOM: D17470). Additionally, this work was equally supported by Grants from Establishment of the Evidence for Clinical Practice-based Korean Medicine Treatment Technologies (K18121) and Development of Foundational Techniques for the Domestic Production of Herbal Medicines (K18405) from the Korea Institute of Oriental Medicine (KIOM), through the Ministry of Science & ICT, Republic of Korea.

References

  1. C. Shenoy, M. B. Patil, R. Kumar, and S. Patil, “Preliminary phytochemical investigation and wound healing activity of Allium cepa linn (Liliaceae),” International Journal of Pharmacy and Pharmaceutical Sciences, no. 2, pp. 167–175, 2009. View at: Google Scholar
  2. R. Thakur, N. Jain, R. Pathak, and S. S. Sandhu, “Practices in Wound Healing Studies of Plants,” Evidence-Based Complementary and Alternative Medicine, vol. 2011, Article ID 438056, 17 pages, 2011. View at: Publisher Site | Google Scholar
  3. H. Imran, M. Ahmad, Atiq-Ur-Rahman et al., “Evaluation of wound healing effects between Salvadora persica ointment and Solcoseryl jelly in animal model,” Pakistan Journal of Pharmaceutical Sciences, vol. 28, no. 5, pp. 1777–1780, 2015. View at: Google Scholar
  4. P. G. Bowler, B. I. Duerden, and D. G. Armstrong, “Wound microbiology and associated approaches to wound management,” Clinical Microbiology Reviews, vol. 14, no. 2, pp. 244–269, 2001. View at: Publisher Site | Google Scholar
  5. J. Boateng and O. Catanzano, “Advanced Therapeutic Dressings for Effective Wound Healing - A Review,” Journal of Pharmaceutical Sciences, vol. 104, no. 11, pp. 3653–3680, 2015. View at: Publisher Site | Google Scholar
  6. K. Järbrink, G. Ni, H. Sönnergren et al., “Prevalence and incidence of chronic wounds and related complications: a protocol for a systematic review,” Systematic Reviews, vol. 5, no. 1, 2016. View at: Publisher Site | Google Scholar
  7. N. Pazyar, R. Yaghoobi, E. Rafiee, A. Mehrabian, and A. Feily, “Skin wound healing and phytomedicine: a review,” Skin Pharmacology and Physiology, vol. 27, no. 6, pp. 303–310, 2014. View at: Publisher Site | Google Scholar
  8. A. C. D. O. Gonzalez, Z. D. A. Andrade, T. F. Costa, and A. R. A. P. Medrado, “Wound healing—a literature review,” Anais Brasileiros de Dermatologia, vol. 91, no. 5, pp. 614–620, 2016. View at: Publisher Site | Google Scholar
  9. M. Rekik, B. S. Khedir, K. K. Moalla, and Z. Sahnoun, “Healing activity of many Tunisian medicinal plants on wounds and burns,” International Journal of Scientific & Engineering Research, vol. 7, no. 5, 2016. View at: Google Scholar
  10. P. Sabale, B. Bhimani, C. Prajapati, and V. Sabalea, “An overview of medicinal plants as wound healers,” Journal of Applied Pharmaceutical Science, vol. 2, no. 11, pp. 143–150, 2012. View at: Google Scholar
  11. C. Agyare, Y. D. Boakye, E. O. Bekoe, A. Hensel, S. O. Dapaah, and T. Appiah, “Review: African medicinal plants with wound healing properties,” Journal of Ethnopharmacology, vol. 177, pp. 85–100, 2016. View at: Publisher Site | Google Scholar
  12. C. O. Okoli, P. A. Akah, and A. S. Okoli, “Potentials of leaves of Aspilia africana (Compositae) in wound care: An experimental evaluation,” BMC Complementary and Alternative Medicine, vol. 7, article no. 24, 2007. View at: Publisher Site | Google Scholar
  13. K. K. Ajibesin, “Ethnobotanical survey of plants used for skin diseases and related ailments in Akwa Ibom State, Nigeria,” Ethnobotany Research and Applications , vol. 10, pp. 463–522, 2012. View at: Google Scholar
  14. I. F. Nwafor, K. M. Tchimene, F. P. Onyekere, and ETAL, “Ethnobiological study of traditional medicine practices for the treatment of chronic leg ulcer in South eastern Nigeria,” Indian Journal of Traditional Knowledge, vol. 17, no. 1, pp. 34–42, 2018. View at: Google Scholar
  15. C. O. Okoli, P. A. Akah, S. V. Nwafor, A. I. Anisiobi, I. N. Ibegbunam, and O. Erojikwe, “Anti-inflammatory activity of hexane leaf extract of Aspilia africana C.D. Adams,” Journal of Ethnopharmacology, vol. 109, no. 2, pp. 219–225, 2007. View at: Publisher Site | Google Scholar
  16. S. E. Ukwueze, A. N. Aghanya, A. A. Mgbahurike et al., “An evaluation of the analgesic and anti-inflammatory activities of the solvent fractions of Aspilia africana (pers.),” World Journal of Pharmacy and Pharmaceutical Sciences, vol. 2, no. 6, pp. 4177–4189, 2013. View at: Google Scholar
  17. T. O. Oyesola, O. A. Oyesola, and C. S. Okoye, “Effects of aqueous extract of Aspilia Africana on reproductive functions of female wistar rats,” Pakistan Journal of Biological Sciences, vol. 13, no. 3, pp. 126–131, 2010. View at: Publisher Site | Google Scholar
  18. U. Okwuonu, D. C. Baxter-Grillo, H. Njoya, and P. T. Iyemene, “proximate and elemental constituents of Aspilia africana (Wild sunflower) flowers,” Journal of Medicinal Plants Studies, vol. 5, no. 4, pp. 22–27, 2017. View at: Google Scholar
  19. O. Etiosa, A. Akeem, and N. Chika, “Phytochemical Studies and GC-MS Analysis of Chloroform Extract of the Leaves of Aspilia africana,” Asian Journal of Physical and Chemical Sciences, vol. 4, no. 3, pp. 1–8, 2018. View at: Publisher Site | Google Scholar
  20. E. M. Ilondu, “Chemical constituents and comparative toxicity of aspilia africana (pers) C. D ADAMS LEAF extracts against two leafspot fungal Isolates of PAW-PAW (Carica papaya L.),” Indian Journal of Science and Technology, vol. 6, no. 9, pp. 5242–5248, 2013. View at: Google Scholar
  21. B. H. Sahib, H. M. Ouda, and S. I. Abaas, “The Wound Healing Activity of (Rue) Ruta graveolens L. Methanolic Extract in Rats,” International Journal of Pharmaceutical Sciences Review and Research, vol. 29, pp. 263–266, 2014. View at: Google Scholar
  22. D. E. Okwu, “Phytochemical and vitamin content of endogenous spices of south eastern Nigeria,” Journal of Sustainability and Agricultural Environment, vol. 6, pp. 30–37, 2004. View at: Google Scholar
  23. S. Ambiga, R. Narayanan, G. Durga, D. Sukumar, and S. Madhavan, “Evaluation of wound healing activity of flavonoids from Ipomoea carnea Jacq,” Ancient Science of Life, vol. XXVI, no. 3, pp. 45–51, 2007. View at: Google Scholar
  24. A. Muralidhar, K. S. Babu, T. R. Sankar, P. Reddanna, and J. Latha, “Wound healing activity of flavonoid fraction isolated from the stem bark of Butea monosperma (lam) in albino wistar rats,” European Journal of Experimental Biology, vol. 3, no. 6, pp. 1–6, 2013. View at: Google Scholar
  25. R. Geethalakshmi, C. Sakravarthi, T. Kritika, M. Arul Kirubakaran, and D. V. L. Sarada, “Evaluation of antioxidant and wound healing potentials of Sphaeranthus amaranthoides Burm.f,” BioMed Research International, vol. 2013, Article ID 607109, 7 pages, 2013. View at: Publisher Site | Google Scholar
  26. S. Lodhi and A. K. Singhai, “Wound healing effect of flavonoid rich fraction and luteolin isolated from Martynia annua Linn. on streptozotocin induced diabetic rats,” Asian Pacific Journal of Tropical Medicine, vol. 6, no. 4, pp. 253–259, 2013. View at: Publisher Site | Google Scholar
  27. F. J. Faleye, “Terpenoid constituents of aspilia Africana [Pers] c.d. adams leaves,” International Journal of Pharmaceutical Sciences Review and Research, vol. 13, no. 1, pp. 138–142, 2012. View at: Google Scholar
  28. M. U. Ekaiko, A. G. Arinze, and C. U. Iwe, “Phytochemical Constituents and Antimicrobial Potency of Aspilia Africana,” International Journal of Life Sciences Research, vol. 4, no. 1, pp. 9–14, 2016. View at: Google Scholar
  29. L. Razika, A. Thanina, C. Nadjiba, B. Narimen, D. Mahdi, and A. Karim, “Antioxidant and wound healing potential of saponins extracted from the leaves of Algerian Urtica dioica L,” Pakistan Journal of Pharmaceutical Sciences, vol. 30, no. 3, pp. 1023–1029, 2017. View at: Google Scholar
  30. Y. S. Kim, I. H. Cho, M. J. Jeong et al., “Therapeutic efect of total ginseng saponin on skin wound healing,” Journal of Ginseng Research, vol. 35, no. 3, pp. 360–367, 2011. View at: Publisher Site | Google Scholar
  31. Y. Kimura, M. Sumiyoshi, K. Kawahira, and M. Sakanaka, “Effects of ginseng saponins isolated from Red Ginseng roots on burn wound healing in mice,” British Journal of Pharmacology, vol. 148, no. 6, pp. 860–870, 2006. View at: Publisher Site | Google Scholar
  32. X. Su, X. Liu, S. Wang et al., “Wound-healing promoting effect of total tannins from Entada phaseoloides (L.) Merr. in rats,” Burns, vol. 43, no. 4, pp. 830–838, 2017. View at: Publisher Site | Google Scholar
  33. L. Chokotho and E. Van Hasselt, “The use of tannins in the local treatment of burn wounds – a pilot study,” Malawi Medical Journal, vol. 17, no. 1, pp. 19-20, 2005. View at: Publisher Site | Google Scholar
  34. A. A. Gbolade, A. Džamic, and P. D. Marin, “Essential oil constituents ofaspilia africana (pers.) C. D. Adams leaf from nigeria,” Journal of Essential Oil Research, vol. 21, no. 4, pp. 348–350, 2009. View at: Publisher Site | Google Scholar
  35. S. Sasidharan, R. Nilawatyi, R. Xavier, L. Y. Latha, and R. Amala, “Wound healing potential of Elaeis guineensis Jacq leaves in an infected albino rat model,” Molecules, vol. 15, no. 5, pp. 3186–3199, 2010. View at: Publisher Site | Google Scholar
  36. K. Yousefi, S. Hamedeyazdan, D. Hodaei et al., “An in vitro ethnopharmacological study on Prangos ferulacea: A wound healing agent,” BioImpacts, vol. 7, no. 2, pp. 75–82, 2017. View at: Publisher Site | Google Scholar
  37. M. Glamoclija, D. Sokovic, D. iljegovic, S. Ristic, D. Ciric, and V. D. Grubiic, “Chemical Composition and Antimicrobial Activity of Echinophora spinosa L. (Apiaceae) Essential Oil Jasmina,” Records of Natural Products, vol. 5, no. 4, pp. 319–323, 2011. View at: Google Scholar
  38. L. Cherrat, L. Espina, M. Bakkali, D. García-Gonzalo, R. Pagán, and A. Laglaoui, “Chemical composition and antioxidant properties of Laurus nobilis L. and Myrtus communis L. essential oils from Morocco and evaluation of their antimicrobial activity acting alone or in combined processes for food preservation,” Journal of the Science of Food and Agriculture, vol. 94, no. 6, pp. 1197–1204, 2014. View at: Publisher Site | Google Scholar
  39. C. Atef, M. Boualem, M. M. Cherif, H. Youcef, and C. Azzedine, “Chemical composition and antimicrobial activity of essential oils in Xerophytic plant Cotula cinerea Del (Asteraceae) during two stages of development: Flowering and fruiting,” Journal of Applied Pharmaceutical Science, vol. 5, no. 3, pp. 29–34, 2015. View at: Google Scholar
  40. T. A. Ibrahim, A. A. El-Hela, H. M. El-Hefnawy, A. M. Al-Taweel, and S. Perveen, “Chemical composition and antimicrobial activities of essential oils of some coniferous plants cultivated in Egypt,” Iranian Journal of Pharmaceutical Research, vol. 16, no. 1, pp. 328–337, 2017. View at: Google Scholar
  41. R. de Cássia da Silveira E Sá, L. N. Andrade, and D. P. de Sousa, “A review on anti-inflammatory activity of monoterpenes,” Molecules, vol. 18, no. 1, pp. 1227–1254, 2013. View at: Publisher Site | Google Scholar
  42. A. Ogunwandea, I. Eresanyaa O, O. Avoseha, T. Oyegokea, O. Ogunmoyeb, and G. Flaminic, “Chemical composition of essential oils from Nigerian plants,” Pelagia Research Library, vol. 3, no. 1, pp. 279–286, 2012. View at: Google Scholar
  43. I. Orhan, E. Küpeli, M. Aslan, M. Kartal, and E. Yesilada, “Bioassay-guided evaluation of anti-inflammatory and antinociceptive activities of pistachio, Pistacia vera L,” Journal of Ethnopharmacology, vol. 105, no. 1-2, pp. 235–240, 2006. View at: Publisher Site | Google Scholar
  44. D.-S. Kim, H.-J. Lee, Y.-D. Jeon et al., “Alpha-pinene exhibits anti-inflammatory activity through the suppression of MAPKs and the NF-κB pathway in mouse peritoneal macrophages,” American Journal of Chinese Medicine, vol. 43, no. 4, pp. 731–742, 2015. View at: Publisher Site | Google Scholar
  45. S. Mahibalan, M. Stephen, R. T. Nethran, R. Khan, and S. Begum, “Dermal wound healing potency of single alkaloid (betaine) versus standardized crude alkaloid enriched-ointment of Evolvulus alsinoides,” Pharmaceutical Biology, vol. 54, no. 12, pp. 2851–2856, 2016. View at: Publisher Site | Google Scholar
  46. M. Tohidi, M. Khayami, V. Nejati, and H. Meftahizade, “Evaluation of antibacterial activity and wound healing of Pistacia atlantica and Pistacia khinjuk,” Journal of Medicinal Plants Research, vol. 5, no. 17, pp. 4310–4314, 2011. View at: Google Scholar
  47. Z. Parveen, S. Nawaz, S. Siddique, and K. Shahzad, “Composition and Antimicrobial Activity of the Essential Oil from Leaves of Curcuma longa L. Kasur Variety,” Indian Journal of Pharmaceutical Sciences, vol. 75, no. 1, p. 117, 2013. View at: Publisher Site | Google Scholar
  48. D. R. Davis and G. R. Graves, “A new leafmining moth (Cameraria cotinivora, Lepidoptera: Gracillariidae) of the American Smoketree (Cotinus obovatus),” Proceedings of the Entomological Society of Washington, vol. 118, no. 2, pp. 244–253, 2016. View at: Publisher Site | Google Scholar
  49. F. Haghdoost, M. M. Baradaran Mahdavi, A. Zandifar, M. H. Sanei, B. Zolfaghari, and S. H. Javanmard, “Pistacia atlantica resin has a dose-dependent effect on angiogenesis and skin burn wound healing in rat,” Evidence-Based Complementary and Alternative Medicine, vol. 2013, Article ID 893425, 8 pages, 2013. View at: Publisher Site | Google Scholar
  50. Q. M. Nunes, Y. Li, C. Sun, T. K. Kinnunen, and D. G. Fernig, “Fibroblast growth factors as tissue repair and regeneration therapeutics,” Peer Journal, vol. 4, p. e1535, 2016. View at: Publisher Site | Google Scholar
  51. M. H. Wan Rozianoor, K. Nurul Nadia, and S. Nurdiana, “Stachytarpheta jamaicensis ethanolic leaf extract as wound healer on alloxan-induced diabetic sprague dawley rats,” Biotechnology : An Indian Journal, vol. 9, no. 11, pp. 460–466, 2014. View at: Google Scholar
  52. R. Serra, R. Grande, L. Butrico et al., “Chronic wound infections: the role of,” Expert Review of Anti-infective Therapy, vol. 13, no. 5, pp. 605–613, 2015. View at: Publisher Site | Google Scholar
  53. W. Lee, E.-R. Woo, and D. G. Lee, “Phytol has antibacterial property by inducing oxidative stress response in Pseudomonas aeruginosa,” Free Radical Research, vol. 50, no. 12, pp. 1309–1318, 2016. View at: Publisher Site | Google Scholar
  54. M. Ghaneian, M. T, H. Jebali A, S. Hekmatimoghaddam, and M. Mahmoudi, “Antimicrobial activity, toxicity and stability of phytol as a novel surface disinfectant,” Environmental Health Engineering and Management Journal, vol. 2, no. 1, pp. 13–16, 2015. View at: Google Scholar
  55. P. Olofsson, M. Hultqvist, I. Hellgren, and R. Holmdah, “Phytol: A Chlorophyll Component with Anti-inflammatory and Metabolic Properties,” Recent Advances in Redox Active Plant and Microbial Products, pp. 345–359, 2014. View at: Google Scholar
  56. N. D. Phatangare, K. K. Deshmukh, and V. D. Murade, “Isolation and Characterization of Phytol from Justicia gendarussa Burm. f.-An Anti-Inflammatory Compound,” International Journal of Pharmacognosy and Phytochemical Research, vol. 9, no. 6, pp. 864–872, 2017. View at: Publisher Site | Google Scholar
  57. R. O. Silva, F. B. Sousa, and S. R. Damasceno, “Phytol, a diterpene alcohol, inhibits the inflammatory response by reducing cytokine production and oxidative stress,” Fundamental Clinical Pharmacology, vol. 28, no. 4, pp. 455–464, 2014. View at: Publisher Site | Google Scholar
  58. L. Xiong, C. Peng, Q.-M. Zhou et al., “Chemical composition and antibacterial activity of essential oils from different parts of Leonurus japonicus houtt,” Molecules, vol. 18, no. 1, pp. 963–973, 2013. View at: Publisher Site | Google Scholar
  59. H. A. Yamani, E. C. Pang, N. Mantri, and M. A. Deighton, “Antimicrobial activity of Tulsi (Ocimum tenuiflorum) essential oil and their major constituents against three species of bacteria,” Frontiers in Microbiology, vol. 7, article 681, 2016. View at: Publisher Site | Google Scholar
  60. S. S. Dahham, Y. M. Tabana, M. A. Iqbal et al., “The anticancer, antioxidant and antimicrobial properties of the sesquiterpene β-caryophyllene from the essential oil of Aquilaria crassna,” Molecules, vol. 20, no. 7, pp. 11808–11829, 2015. View at: Publisher Site | Google Scholar
  61. M. C. Selestino Neta, C. Vittorazzi, A. C. Guimarães et al., “Effects of β-caryophyllene and,” Pharmaceutical Biology, vol. 55, no. 1, pp. 190–197, 2016. View at: Publisher Site | Google Scholar
  62. L. Halcón and K. Milkus, “Staphylococcus aureus and wounds: a review of tea tree oil as a promising antimicrobial,” American Journal of Infection Control, vol. 32, no. 7, pp. 402–408, 2004. View at: Publisher Site | Google Scholar
  63. B. Bakir, H. Aydin, Ö. Hanefi, E. Düz, and M. Tütüncü, “Investigation of the anti-inflammatory and analgesic activities of β-caryophyllene,” International Journal of Essential Oil Therapeutics, vol. 2, pp. 41–44, 2008. View at: Google Scholar
  64. O. O. Oko, E. Agiang, and E. E. Osim, “Pharmacognosy of Aspilia africana plant: Phytochemistry and Activities,” in Bioactive Phytochemicals: Perspectives for Modern Medicine, Kumar, Ed., pp. 383–409, ASTRAL, 2nd edition, 2014. View at: Google Scholar
  65. C. Obute and O. G. Adubor, “Chemicals detected in plants used for folk medicine in South Eastern Nigeria,” Ethnobotanical Leaflets, vol. 11, pp. 173–194, 2007. View at: Google Scholar
  66. C. Taziebou Lienou, F.-X. Etoa, B. Nkegoum, C. A. Pieme, and D. P. D. Dzeufiet, “Acute and subacute toxicity of Aspilia africana leaves,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 4, no. 2, pp. 127–134, 2007. View at: Google Scholar
  67. C. Okwuonu, K. Oluyemi, B. Grillo et al., “Effects Of Methanolic extract of Aspilia africana leaf on the ovarian tissues and weights of Wistar rats,” The Internet Journal of Alternative Medicine, vol. 5, no. 1, 2006. View at: Google Scholar
  68. O. O. K. Oko, E. A. Agiang, E. E. Osim, and O. R. Asuquo, “Toxicological evaluation of aspilia africana leaf extracts in mice,” American Journal of Pharmacology and Toxicology, vol. 6, no. 3, pp. 96–101, 2011. View at: Publisher Site | Google Scholar
  69. A. M. Eluwa, E. Q. Imossan, and R. O. Asuquo, “Pre-natal exposure and toxicity of aqueous leaf extract of Aspilia africana on placenta of albino wistar rat foetuses,” European Journal of Pharmaceutical and Medical Research, vol. 4, no. 9, pp. 46–51, 2017. View at: Google Scholar
  70. O. Kayode A., O. Uche C., B. D. Grillo, and O. Tolulope O., “Toxic effects of methanolic extract of Aspilia africana leaf on the estrous cycle and uterine tissues of Wistar rats,” International Journal of Morphology, vol. 25, no. 3, pp. 609–614, 2007. View at: Google Scholar
  71. A. O. Eweka, “Histological studies of the effects of oral administration of Aspilia africana (Asteraceae) leaf extract on the ovaries of female wistar rats,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 6, no. 1, pp. 57–61, 2009. View at: Google Scholar
  72. O. R. Asuquo, M. A Eluwa, and O. E. Mesembe, “Antispermatogenic activity of Aspilia africana methanol leaf extract in male Wistar rats,” British Journal of Medicine & Medical Research, vol. 6, no. 4, pp. 415–422, 2015. View at: Google Scholar
  73. A. Eweka, “Anti-ulcer effect of Aspilia africana (Asteraceae) leaf extract on induced duodenal ulcer of adult Wistar rats (Rattus Norvegicus) – A Histological Study,” The Internet Journal of Alternative Medicine, vol. 8, no. 1, 2008. View at: Google Scholar
  74. T. B. Nguelefack, P. Watcho, S. L. Wansi et al., “Short Communication: The antiulcer effects of the methanol extract of the leaves of Aspilia africana (Asteraceae) in rats,” African Journal of Traditional, Complementary and Alternative Medicines, vol. 2, no. 3, pp. 233–237, 2005. View at: Publisher Site | Google Scholar
  75. A. A. Attama, P. F. Uzor, C. O. Nnadi, and C. G. Okafor, “Evaluation of the wound healing activity of gel formulations of leaf extract of Aspila Africana fam. Compositae,” Journal of Chemical and Pharmaceutical Research, vol. 3, no. 3, pp. 718–724, 2011. View at: Google Scholar
  76. E. A. Osunwoke, O. Otakore, and S. Lelei, “Wound Healing Effects Of The Leaves Extract Of Aspilia africana On Wistar Rats (Rattus norvegicus),” Vedic Research International Phytomedicine, vol. 2, no. 1, pp. 1–5, 2014. View at: Publisher Site | Google Scholar
  77. K. O. Ajeigbe, S. S. Enitan, D. R. Omotoso, and O. O. Oladokun, “Acute effects of aqueous leaf extract of Aspilia africana C.D. Adams on some haematological parameters in rats.,” African journal of traditional, complementary, and alternative medicines : AJTCAM / African Networks on Ethnomedicines, vol. 10, no. 5, pp. 236–243, 2013. View at: Google Scholar
  78. M. Mahre, B. Umaru, S. Ngulde et al., “Haematological changes and wound healing effects of sildenafil citrate in diabetic albino rats,” Sokoto Journal of Veterinary Sciences, vol. 15, no. 1, pp. 20–26, 2017. View at: Publisher Site | Google Scholar
  79. I. Tumen, I. Süntar, H. Keleş, and E. Küpeli Akkol, “A therapeutic approach for wound healing by using essential oils of cupressus and Juniperus species growing in Turkey,” Evidence-Based Complementary and Alternative Medicine, vol. 2012, Article ID 728281, 7 pages, 2012. View at: Publisher Site | Google Scholar
  80. C. E. Johnson, A. O. Eseyin, S. A. Udobre et al., “Antibacterial Effect of Methanolic Extract of the Root of Aspilia africana,” Nigerian Journal of Pharmaceutical and Applied Science Research, vol. 1, no. 1, pp. 44–50, 2011. View at: Google Scholar
  81. R. B. Agbor, I. A. Ekpo, and B. E. Ekanem, “Antimicrobial Properties and Nutritional Composition of Aspilia africana C.D. Adams,” International Journal of Applied Science and Technolog, vol. 2, no. 7, 2012. View at: Google Scholar
  82. S. R. Pawar and A. F. Toppo, “Plants that heal wounds. A review,” Herba Polonica, vol. 58, no. 1, 2012. View at: Google Scholar
  83. B. S. Sherah, U. E. Onche, J. I. Mbonu et al., “Antimicrobial activity and chemical composition of flowers of Aspilia africana,” Advances in Life Sciences and Technology, vol. 16, 2014. View at: Google Scholar
  84. O. Ezeigbo, D. Awomukwu, and I. Ezeigbo, “The Antimicrobial and Phytochemical Analysis of the Leaves of Aspilia africana on Clinical Isolates,” European Journal of Medicinal Plants, vol. 15, no. 2, pp. 1–6, 2016. View at: Publisher Site | Google Scholar
  85. I. I. Anibijuwon, O. P. Duyilemi, and A. K. Onifade, “Antimicrobial activity of leaf of Aspila africana on some pathogenic organisms of clinical origin,” Nigerian Journal of Microbiology, vol. 24, no. 1, pp. 2048–2055, 2010. View at: Google Scholar
  86. U. Essiett and I. Unung, “Comparative phytochemical and physico-chemical properties of Aspilia africana (Pers) C. D. Adams and Tithonia diversifolia (Hemsl) A. Gray petals as a scientific backing to their tradomedicinal potentials,” International Journal of Modern Biology and Medicine, vol. 3, no. 2, pp. 88–100, 2013. View at: Google Scholar
  87. D. E. Okwu and C. Josiah, “Evaluation of the chemical composition of two Nigerian medicinal plants,” African Journal of Biotechnology, vol. 5, no. 4, pp. 357–361, 2006. View at: Google Scholar
  88. T. Abii and E. Onuoha, “The Chemical Constituents of the Leaf of Aspilia africana as a Scientific Backing to its Tradomedical Potentials,” Agricultural Journal, vol. 6, no. 1, pp. 28–30, 2011. View at: Publisher Site | Google Scholar
  89. B. H. Porras-Reyes, T. A. Mustoe, W. H. Lewis, J. Roman, and L. Simchowitz, “Enhancement of wound healing by the alkaloid taspine defining mechanism of action,” Proceedings of the Society for Experimental Biology and Medicine, vol. 203, no. 1, pp. 18–25, 1993. View at: Publisher Site | Google Scholar
  90. J. Fetse, J. Kyekyeku, E. Dueve, and K. Mensah, “Wound Healing Activity of Total Alkaloidal Extract of the Root Bark of Alstonia boonei (Apocynacea),” British Journal of Pharmaceutical Research, vol. 4, no. 23, pp. 2642–2652, 2014. View at: Publisher Site | Google Scholar
  91. M. J. Chavan, P. S. Wakte, and D. B. Shinde, “Analgesic and anti-inflammatory activity of Caryophyllene oxide from Annona squamosa L. bark,” Phytomedicine, vol. 17, no. 2, pp. 149–151, 2010. View at: Publisher Site | Google Scholar
  92. A. Vijayalaxmi, V. Bakshi, N. Begum, V. Kowmudi, Y. Kumar, and Y. Yogesh Reddy, “Anti-Arthritic and Anti Inflammatory Activity of Beta Caryophyllene against Freunds Complete Adjuvant Induced Arthritis in Wistar Rats,” Journal of Bone Reports Recommendations, vol. 1, no. 2, pp. 10–4172, 2015. View at: Google Scholar
  93. S. S. Dahham, M. Y. Tabana, and K. B. Ahamed, “In vivo anti-inflammatory activity of β-caryophyllene, evaluated by molecular imaging,” Molecules & Medicinal Chemistry, vol. 1: e1001, 2015. View at: Publisher Site | Google Scholar
  94. J.-R. Kuiate, P.-H. Amvam Zollo, G. Lamaty, C. Menut, and J.-M. Bessière, “Composition of the essential oils from the leaves of two varieties of Aspilia africana (Pers.) C. D. Adams from Cameroon,” Flavour and Fragrance Journal, vol. 14, no. 3, pp. 167–169, 1999. View at: Publisher Site | Google Scholar
  95. L. Usman, I. A. Oladosu, N. Olawore et al., “Chemical composition of leaf oil of Nigerian grown Aspilia africana.C.D. Adams,” Adams. American-Eurasian Journal of Sustainable Agriculture, vol. 3, no. 4, pp. 899–901, 2009. View at: Google Scholar
  96. L. T. H. Tan, L. H. Lee, W. F. Yin et al., “Traditional Uses, Phytochemistry, and Bioactivities of Cananga odorata (Ylang-Ylang),” Evidence-Based Complementary and Alternative Medicine, vol. 2015, Article ID 896314, 30 pages, 2015. View at: Publisher Site | Google Scholar
  97. C. Pérez Zamora, C. Torres, and M. Nuñez, “Antimicrobial Activity and Chemical Composition of Essential Oils from Verbenaceae Species Growing in South America,” Molecules, vol. 23, no. 3, p. 544, 2018. View at: Publisher Site | Google Scholar
  98. B. Bayala, I. H. Bassole, C. Gnoula et al., “Chemical Composition, Antioxidant, Anti-Inflammatory and Anti-Proliferative Activities of Essential Oils of Plants from Burkina Faso,” PLoS ONE, vol. 9, no. 3, p. e92122, 2014. View at: Publisher Site | Google Scholar
  99. P. Sitarek, P. Rijo, C. Garcia et al., “Antibacterial, Anti-Inflammatory, Antioxidant, and Antiproliferative Properties of Essential Oils from Hairy and Normal Roots of Leonurus sibiricus L. And Their Chemical Composition,” Oxidative Medicine and Cellular Longevity, vol. 2017, Article ID 7384061, 2017. View at: Publisher Site | Google Scholar
  100. J. Cárdenas, J. Rojas, L. Rojas-Fermin, M. Lucena, and A. Buitrago, “Essential oil composition and antibacterial activity of Monticalia greenmaniana (Asteraceae),” Natural Product Communications (NPC), vol. 7, no. 2, pp. 243-244, 2012. View at: Google Scholar
  101. A. Kadri, I. B. Chobba, Z. Zarai et al., “Chemical constituents and antioxidant activity of the essential oil from aerial parts of Artemisia herba-alba grown in Tunisian semi-arid region,” African Journal of Biotechnology, vol. 10, no. 15, pp. 2923–2929, 2011. View at: Publisher Site | Google Scholar
  102. C. El-Kalamouni, P. Venskutonis, B. Zebib, O. Merah, C. Raynaud, and T. Talou, “Antioxidant and Antimicrobial Activities of the Essential Oil of Achillea millefolium L. Grown in France,” Medicines, vol. 4, no. 2, p. 30, 2017. View at: Publisher Site | Google Scholar
  103. J.-Y. Lee, Y.-S. Kim, and D.-H. Shin, “Antimicrobial synergistic effect of linolenic acid and monoglyceride against Bacillus cereus and Staphylococcus aureus,” Journal of Agricultural and Food Chemistry, vol. 50, no. 7, pp. 2193–2199, 2002. View at: Publisher Site | Google Scholar
  104. R. Reifen, A. Karlinsky, A. H. Stark, Z. Berkovich, and A. Nyska, “α-Linolenic acid (ALA) is an anti-inflammatory agent in inflammatory bowel disease,” The Journal of Nutritional Biochemistry, vol. 26, no. 12, pp. 1632–1640, 2015. View at: Publisher Site | Google Scholar
  105. J. Ren and S. H. Chung, “Anti-inflammatory effect of α-linolenic acid and its mode of action through the inhibition of nitric oxide production and inducible nitric oxide synthase gene expression via NF-κB and mitogen-activated protein kinase pathways,” Journal of Agricultural and Food Chemistry, vol. 55, no. 13, pp. 5073–5080, 2007. View at: Publisher Site | Google Scholar

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