DNA Markers to Discriminate Cannabis sativa L. ‘Cheungsam’ with Low Tetrahydrocannabinol (THC) Content from Other South Korea Cultivars Based on the Nucleotide Sequences of Tetrahydrocannabinolic Acid Synthase and Putative 3-Ketoacyl-CoA Synthase Genes

Figure 1

Multiple alignment of the partial nucleotide sequences of tetrahydrocannabinolic acid (THCA) synthase genes from samples listed in Table 1. The dots indicate the consensus nucleotide; bold arrows indicate the primers used to amplify DNA markers of CH and the internal standard; the boxes represent nucleotide sequences and the positions of primers CHF3, CHR2, and ISR. (a)–(c) represent residues that might encode the regions located in the substrate-binding pocket beside to the FAD cofactor, and (d)–(f) represent residues that might encode the regions located on the surface of THCA synthase. The regions encoded by residues were predicted on the basis of the uncovered crystal structure of THCA synthase (PDB ID 3VTE) from Cannabis sativa L.