Research Article
Antiproliferative and Proapoptotic Effects of a Protein Component Purified from Aspongopus chinensis Dallas on Cancer Cells In Vitro and In Vivo
Table 2
Proteins identified with high confidence from the CHP of A. chinensis Dallas.
| Peptide sequence | Prec MW | Prec m/z | Theor MW | Theor m/z | Protein |
| DTLFEYLENPKK | 1552.774414 | 777.3945 | 1552.777344 | 777.3959351 | Cytochrome c | MVFAGIK | 807.4334717 | 404.724 | 807.4312744 | 404.7229309 | LENPKK | 727.4298706 | 364.7222 | 727.4228516 | 364.718689 | ADLIAYLEQATK | 1391.730103 | 696.8723 | 1391.729614 | 696.8720703 | DTLFEYLENPKK | 1495.751831 | 748.8832 | 1495.755859 | 748.8851929 | KPQERADLIAYLEQATK | 2032.07959 | 678.3671 | 2032.05896 | 678.3602295 | VGPNLSGLIGR | 1139.647217 | 570.8309 | 1139.629883 | 570.8222046 |
| EGLGIFVLDK | 1090.612305 | 546.3134 | 1089.607056 | 545.810791 | Ferritin | QNFHADSEEAINK | 1501.667236 | 751.8409 | 1501.679688 | 751.847168 | SLGDLLTNVR | 1086.604492 | 544.3095 | 1086.603271 | 544.30896 | VKEGLGIFVLDK | 1316.761719 | 659.3881 | 1316.770386 | 659.3924561 | AVEAALQLEK | 1070.595215 | 536.3049 | 1070.597168 | 536.3058472 | LAFVEIDR | 977.5206909 | 489.7676 | 977.5181885 | 489.7663879 | TLALLHR | 822.5070801 | 412.2608 | 822.5075684 | 412.2610474 | LFNLFYFAK | 1162.605713 | 582.3101 | 1162.606323 | 582.3104248 |
| MISLCGPLSIIGR | 1431.728638 | 716.8716 | 1431.757813 | 716.8861694 | Superoxide dismutase | TIVVHADPDDLGK | 1379.704834 | 690.8597 | 1378.709229 | 690.3618774 | VACGVIGITK | 1073.583618 | 537.7991 | 1073.590332 | 537.8024292 | HVGDLGNICAEANGVAK | 1752.820435 | 877.4175 | 1752.846436 | 877.430542 | YDTPELVEK | 1149.551025 | 575.7828 | 1149.55542 | 575.7849731 | AGGQVAWTK | 916.4700928 | 459.2423 | 916.4766846 | 459.2456055 | EAFSLFDKDGDGTITTK | 1843.888916 | 615.6369 | 1843.884033 | 615.6352539 |
| LFDLFFFAK | 1203.633667 | 602.8241 | 1203.632813 | 602.8236694 | Secreted Hemocyanin-like protein |
| AVGVDLNLK | 927.5370483 | 464.7758 | 927.5389404 | 464.7767334 | Glutathione s-transferase |
| KASGQKIGQK | 1044.60791 | 523.3112 | 1044.592773 | 523.3036499 | Tyrosine-protein phosphatase corkscrew isoform x2 | LVSLSEQQLIDCSR | 1704.82605 | 853.4203 | 1704.835205 | 853.4249268 |
| LVSLSEQQLIDCSR | 1704.82605 | 853.4203 | 1704.835205 | 853.4249268 | Cysteine protease |
| INNPLK | 698.3964844 | 350.2055 | 698.3963013 | 350.2054138 | Death-associated protein 1 |
| KLNTNSLQK | 1044.60791 | 523.3112 | 1044.592773 | 523.3036499 | Telomerase reverse transcriptase |
| VLHGTVVAVGPGAR | 1331.76123 | 444.9277 | 1331.767334 | 444.929718 | Heat shock protein, putative |
| LDEALEAAR | 986.5010986 | 494.2578 | 986.5032959 | 494.2589111 | TATA element modulatory factor |
| RAKEASAAEGGIK | 1286.706421 | 644.3605 | 1286.694214 | 644.3544312 | 6S proteasome non-ATPase regulatory subunit 4 |
| SILSTLYASEVMYENT | 1819.820435 | 607.6141 | 1819.85498 | 607.6256104 | ATP synthase subunit a | SLYNEFKLLTGPNNK | 1736.867798 | 435.2242 | 1736.90979 | 435.2347107 | LLTGPNNK | 856.4470825 | 429.2308 | 856.4654541 | 429.2399902 | INIIMNNMLNSLYNEFK | 2070.905273 | 1036.46 | 2071.011719 | 1036.513184 | TNLFSTFDPATSIK | 1541.681274 | 771.8479 | 1541.761353 | 771.8879395 | PFMVLIETISNLIR | 1644.788452 | 549.2701 | 1644.927246 | 549.3164063 | STLYASEVMYE | 1291.598267 | 646.8064 | 1291.564209 | 646.7893677 | INIIMN | 716.3768921 | 359.1957 | 716.3890991 | 359.2018127 | MTNLFSTFDPAT | 1343.644287 | 672.8294 | 1343.606812 | 672.8106689 |
| MVFIMFWFIWVR | 1673.763306 | 837.8889 | 1673.86145 | 837.9379883 | | LMYLTWK | 953.5025024 | 477.7585 | 953.5044556 | 477.7595215 | TGLMGLLQPFADGLK | 1559.709106 | 780.8618 | 1559.838135 | 780.9263306 | | SVLGYIQLRK | 1176.579468 | 589.297 | 1176.686646 | 589.3505859 | NADH-ubiquinone oxidoreductase chain 1 | IFMSFPLFF | 1147.543457 | 574.779 | 1147.577637 | 574.7960815 | | MSFPLFFCWLSSCLAETNR | 2364.968506 | 592.2494 | 2365.069336 | 592.2745972 | | SEYMNIIFM | 1146.536621 | 574.2756 | 1146.508911 | 574.2617798 | |
| FQGNKFYPLNK | 1355.621094 | 678.8178 | 1355.687378 | 678.8509521 | Cytochrome b | DVNNGWLMR | 1104.517456 | 553.266 | 1104.502197 | 553.2583618 | AGRGLYYGSYK | 1233.599121 | 617.8068 | 1233.614258 | 617.8143921 | WLWGGFSVDNATLTR | 1722.773315 | 862.3939 | 1722.836548 | 862.4255371 | SNYDKTPFHPYF | 1514.661133 | 758.3378 | 1514.682983 | 758.3488159 | MLGDPENFIP | 1147.543457 | 574.779 | 1147.521973 | 574.7682495 |
| ISFINQNIK | 1075.515259 | 538.7649 | 1075.602539 | 538.8085938 | NADH dehydrogenase subunit 6 |
| NDIICSQMNWK | 1407.588623 | 704.8016 | 1407.627563 | 704.8210449 | ATP synthase protein 8 |
| TASYDREK | 968.3950806 | 485.2048 | 968.4562988 | 485.2354431 | NADH-ubiquinone oxidoreductase chain 3 | TICSIISK | 920.4076538 | 461.2111 | 920.5001221 | 461.2573242 | MMMYVMMTTI | 1266.534912 | 634.2747 | 1266.519043 | 634.2667847 |
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