Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)

Figure 2

Sesamin (SSM) inhibits the mRNA and protein expression of T0901317 (T090)-induced LXRα downstream genes. Differentiated HepaRG cells treated with 20 or 40 μM SSM alone or in combination with 10 μM T090 for 24 h. (a) Quantitative real-time PCR results of gene expression levels of SREBP‐1c, FAS, SCD, ACC, ACLY, and FAE. β-actin was used as an internal control. Data represent the mean ± SE; n = 3; ^###; compared with control or T090-treated groups, respectively, as indicated. (b) and (c) Differentiated HepaRG cells treated with SSM and T090, either individually or in combination for 24 h, were harvested, and their protein expression levels were analyzed via western blot. Quantitation of the indicated protein bands was corrected by β-actin expression. The representative blot shown was quantified with ImageJ software. Data represent the mean ± SE; n = 3; ; compared with control or T090-treated groups, respectively, as indicated.

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