Sesamin, a Naturally Occurring Lignan, Inhibits Ligand-Induced Lipogenesis through Interaction with Liver X Receptor Alpha (LXRα) and Pregnane X Receptor (PXR)

Figure 4

Sesamin (SSM) selectively induces reverse cholesterol transport- (RCT-) related genes in LS174T cells, affects AMPK signaling pathway, and differentially affects hepatocytes and intestinal cells. (a) and (b) LS174T cells were treated with SSM alone or in combination with T090 or compound C for 24 h. The expressions of RCT-related genes, ABCA1 and ABCG1, SREBP-1c, FAS, and SCD were analyzed by real-time PCR. Data represent the mean ± SE; n = 3; compared with control or T090-treated groups as indicated. (c) Differentiated HepaRG cells or LS174T cells treated with SSM and T090, either individually or in combination for 24 h were harvested, and their protein levels of phospho-AMPK and β-actin (internal control) were analyzed via western blot. Quantitation of the indicated protein bands was corrected by β-actin expression. The representative blot shown was quantified with ImageJ software. Data represent the mean ± SE; n = 3; ; ; compared with the control group as indicated. (d) DNA binding affinity assay (DAPA) analysis at the SREBP-1c (upper) and ABCG1 (lower) response element from HepaRG and LS1714T cells. Five hundred micrograms of nuclear extracts, from SSM treatment in combination with 10 μM T090, were used in DAPA experiments. A representative blot is presented, and the protein expression in untreated control cells was set to 1.

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