Qualitative and Quantitative Analysis for the Chemical Constituents of Tetrastigma hemsleyanum Diels et Gilg Using Ultra-High Performance Liquid Chromatography/Hybrid Quadrupole-Orbitrap Mass Spectrometry and Preliminary Screening for Anti-Influenza Virus Components

Table 1

Identification analysis of chemical constituents of T. hemsleyanums in negative ion mode of mass spectrometry.

No.	tR (min)	Name	Formula	Detected m/z	Expected m/z	M - X	Error (ppm)	Fragmentor information
1	0.9	Procyanidin dimmer		577.1334	577.1341	M – H	-0.0007	125.0228, 151.0386
								161.0230, 203.0706
								289.0716, 407.0767
2	1.15	Neochlorogenic acid		353.0331	353.0867	M – H	-0.0536	135.0434, 161.0591
								173.0598, 179.0337
								191.0553
3	1.49	Chlorogenic acid		353.0337	353.0867	M – H	-0.0530	135.0436, 161.0231
								173.0443,191.0549
4	1.5	Protocatechuic acid glucoside		315.0717	315.0711	M – H	0.0006	108.0200, 109.0279
								152.0100, 153.0179
5	1.52	Gallic acid		169.0119	169.0132	M – H	-0.0013	125.0228, 145.3992
								151.0022
6	1.67	Catechins		289.2617	289.2613	M – H	0.0004	121.0280, 125.0230
								137.0231, 161.0597
								187.0391, 245.0817
7	2.04	Epicatechin		289.2618	289.2613	M – H	0.0005	125.0228, 161.0595
								179.0337, 187.0391
								245.0813, 271.0609
8	2.74	Isorhamnetin-3-pyranosearabinose-7-glucosyl-rhamnoside		755.6536	755.6526	M – H	0.0010	299.0200, 313.0343
								314.0414, 609.1453
9	2.93	4-hydroxy-3-methoxybenzaldehyde		151.0389	151.0390	M – H	-0.0001	77.0397, 93.0328
								109.0279, 121.0275
								136.0150, 137.0217
								151.0389
10	3.68	Kaempferol-3-o-furananose-7-O-rhamnosyl-glucoside		725.6232	725.6266	M – H	-0.0034	255.0300, 283.0253
								284.0330, 285.0393
								575.1413
11	4	Vitexin-rhamnoside		577.5144	577.5124	M – H	0.0020	283.0609, 311.0560
								341.0664, 353.0664
								413.0873, 457.1156
12	4.18	Rutin		609.5162	609.5112	M – H	0.0050	211.0394, 255.0299
								256.0332, 283.0238
								301.0348
13	4.23	Kaempferol-3-O-furana-nose-7-O-rhamnose isomer		725.6237	725.6266	M – H	-0.0029	255.0295, 284.0325
								431.1031
14	4.86	Isoquercetin		463.3790	463.3697	M – H	0.0093	151.0025, 243.0296
								255.0298, 271.0250
								300.0278
15	5.5	Oxidized resveratrol		243.0656	243.0652	M – H	0.0004	157.0644, 160.0471
								175.0752, 197.0597
								201.0548, 225.0549
16	6.27	Salicylic acid		137.0229	137.0233	M – H	-0.0004	93.0329, 108.0198
								119.7794
17	6.53	Quercitrin		447.3834	447.3703	M – H	0.0131	151.0029, 255.0296
								301.0356, 426.9522
18	6.94	Kaempferol-3-O-rutino-side		593.5119	593.5118	M – H	0.0001	255.0299, 267.0291
								285.0405
19	7.9	Astragalin		447.3738	447.3703	M – H	0.0035	227.0346, 255.0299
								284.0329, 285.0398
20	19.27	Quercetin		301.0352	301.0343	M – H	0.0009	121.0279, 149.0229
								151.0022, 161.0227
								178.9973, 245.0449
21	19.77	Vitexin		431.0972	431.0973	M – H	-0.0001	269.0262, 283.0627
								311.0527, 341.0764
22	20.12	Emodin-8-O-β-D-glucoside		431.1557	431.0973	M – H	0.0584	181.0859, 225.1121
								269.1029, 413.1434
23	20.98	Apigenin		269.0454	269.0445	M – H	0.0009	159.0439, 181.0648
								213.0542, 225.0545
								227.0343, 241.0497
24	21.4	Kaempferol		285.2303	285.2295	M – H	0.0008	159.0440, 183.0448
								211.0394, 227.0340
								239.0346
25	21.64	Isorhamnetin		315.2507	315.2555	M – H	-0.0048	151.0023, 163.0020
								271.0254, 283.0234
								300.0273
26	22.19	Rock acid		357.0607	357.0605	M – H	0.0002	112.6701, l21.0279
								163.0184
27	27.41	Gallocatechin		305.2661	305.2607	M – H	0.0054	125.0962, 167.5970
								287.1647
28	27.62	Gingergly eolipid A		675.3498	675.3586	M – H	-0.0088	161.0440, 277.2170
								397.1349, 415.14514
29	27.9	Epigallocatechin		305.0750	305.0656	M – H	0.0094	125.0957, 137.0593
								179.1062, 287.1654
30	29.26	Gingergly eolipid B		677.3754	677.3743	M – H	0.0011	125.0230, 161.0444
								179.0553, 279.2327
								397.1341, 415.1567
31	29.95	1-linoleylglycero-2-phospho-ethanolanine		476.5684	476.5653	M – H	0.0031	140.0180, 196.0372
								214.8795, 279.2330
32	38.06	Lysophosphatidic acid		435.2514	435.2506	M – H	0.0008	78.9573, 152.9945
33	42.19	Soya-cerebroside I		712.5367	712.5358	M – H	0.0009	179.0551, 271.2278
								278.2481, 296.2603
								532.4733, 550.4841
34	42.9	Linoleic acid phosphatidic acid		433.4959	433.4974	M – H	-0.0015	78.9574, 152.9946
35	45.84	4-(2-dodecyl)-benzene-sulfonate		325.4846	325.4884	M – H	-0.0038	119.0488, 170.0033
								183.0112
36	51.25	4-(1-methyl-dodecyl)-benzene-sulfonic acid		339.5191	339.5150	M – H	0.0041	79.9554, 119.0487
								170.0030, 225.0585
								239.0742