Dihydroartemisinin Inhibits Proliferation and Induces Apoptosis of Human Hepatocellular Carcinoma Cell by Upregulating Tumor Necrosis Factor via JNK/NF-κB Pathways

Figure 5

DHA induced TNF synthesis via JNK/NF-κB pathways. (a) Western blotting of JNK, c-JUN, and MAP3K8 of the cells with DHA treatment for 24 and 48 hours. (b, c) The semiquantification of phosphorylated JNK, phosphorylated c-JUN, and MAP3K8. (d) Western blotting of TNF-α, TRAF6, nuclear p65, and RELA. (e, f) The semiquantification of the target proteins ratio to GAPDH (TNF-α and TRAF6) and ratio to RELA (nuclear p65). were accepted as significant difference. (g) Graphical abstract of the involving pathways.

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