Migration and Proliferation Effects of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) and Thymoquinone (TQ) on In Vitro Wound Healing Models

Figure 4

(a) Scratch assay images of serum-starved 3T3-L1 cells treated with TQ-NLC. The wound was completely closed at 3 μM and 6 μM concentration of TQ-NLC. At 24 hours, at least 50% of the gap was closed. The arrow shows the wound gap size. Wound closure rates are expressed as percentage of scratch closure after 48 hours compared with the initial area. Scale bar, 5000 μM (100x magnification). (b) Scratch assay images of serum-starved 3T3-L1 cells treated with TQ. The wound was completely closed at 3 μM concentration of TQ. At 24 hours, at least 50% of the gap was closed. The arrow shows the wound gap size. Wound closure rates are expressed as percentage of scratch closure after 48 hours compared with the initial area. Scale bar, 5000 μM (100x magnification). (c) Scratch assay images of serum-starved 3T3 cells treated with TQ-NLC. The wound was completely closed at all concentrations of TQ-NLC at 48 hours. At 24 hours, at least 50% of the gap was closed. The arrow shows the wound gap size. Wound closure rates are expressed as percentage of scratch closure after 48 hours compared with the initial area. Scale bar, 5000 μM (100x magnification). (d) Scratch assay images of serum-starved 3T3 cells treated with TQ. The wound was completely closed at all concentrations of TQ at 48 hours. At 24 hours, at least 50% of the gap was closed. The arrow shows the wound gap size. Wound closure rates are expressed as percentage of scratch closure after 48 hours compared with the initial area. Scale bar, 5000 μM (100x magnification).

(a)

(b)

(c)

(d)