Migration and Proliferation Effects of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) and Thymoquinone (TQ) on In Vitro Wound Healing Models
Table 2
Percentage of healthy, apoptotic, and necrotic cells of 3T3 treated with TQ-NLC as determined by flow cytometry.
24 h
48 h
72 h
Control
Healthy cells
91.7 ± 0.1
89.4 ± 0.0
86.7 ± 1.2
Early apoptosis
0.9 ± 0.1
1.2 ± 0.1
2.3 ± 0.6
Late apoptosis
6.6 ± 0.1
8.7 ± 0.1
9.9 ± 0.6
Necrosis
0.8 ± 0.1
0.7 ± 0.0
1.1 ± 0.1
NLC
Healthy cells
90.1 ± 0.2
89.9 ± 0.1
87.4 ± 0.0
Early apoptosis
2.0 ± 0.1
2.2 ± 0.2
2.7 ± 0.3
Late apoptosis
4.6 ± 0.1
4.5 ± 0.2
5.8 ± 0.3
Necrosis
3.3 ± 0.1
3.4 ± 0.4
4.1 ± 0.1
3 μM
Healthy cells
92.1 ± 0.8
89.3 ± 1.5
95.1 ± 0.8
Early apoptosis
1.5 ± 0.3
0.9 ± 0.2
1.3 ± 0.2
Late apoptosis
5.0 ± 0.6
8.3 ± 1.2
2.7 ± 0.4
Necrosis
1.5 ± 0.4
1.5 ± 0.5
0.9 ± 0.2
6 μM
Healthy cells
89.9 ± 0.2
92.8 ± 0.1
93.6 ± 0.9
Early apoptosis
0.9 ± 0.1
0.6 ± 0.1
1.8 ± 0.1
Late apoptosis
7.7 ± 0.3
4.9 ± 0.2
3.9 ± 0.1
Necrosis
1.5 ± 0.0
1.8 ± 0.4
0.7 ± 0.7
12 μM
Healthy cells
93.6 ± 2.0
94.7 ± 0.2
93.2 ± 0.7
Early apoptosis
0.2 ± 1.1
0.5 ± 0.1
1.1 ± 0.1
Late apoptosis
0.8 ± 2.4
3.8 ± 0.0
4.4 ± 0.6
Necrosis
5.4 ± 1.5
1.0 ± 0.0
1.3 ± 0.0
Each data point represents the mean of three independent experiments. Each value was represented as mean ± SEM.
