Migration and Proliferation Effects of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) and Thymoquinone (TQ) on In Vitro Wound Healing Models

Table 4

Percentage of healthy, apoptotic, and necrotic cells of 3T3-L1 treated with TQ as determined by flow cytometry.

		24 h	48 h	72 h
Control	Healthy cells	86.7 ± 1.8	84.7 ± 4.2	84.4 ± 2.4
	Early apoptosis	3.3 ± 0.1	1.1 ± 0.1	4.2 ± 0.4
	Late apoptosis	6.2 ± 0.8	10.3 ± 0.5	9.2 ± 1.7
	Necrosis	3.8 ± 0.9	3.9 ± 0.1	4.2 ± 0.3
3 μM	Healthy cells	89.3 ± 0.3	80.6 ± 3.7	86.4 ± 2.1
	Early apoptosis	2.9 ± 0.3	1.2 ± 0.5	3.5 ± 0.6
	Late apoptosis	6.6 ± 0.4	15.3 ± 3.6	8.0 ± 1.3
	Necrosis	1.2 ± 0.4	2.9 ± 0.5	2.1 ± 0.3
6 μM	Healthy cells	87.6 ± 2.1	82.5 ± 3.0	82.1 ± 3.1
	Early apoptosis	2.8 ± 1.1	1.2 ± 0.5	3.8 ± 0.5
	Late apoptosis	6.3 ± 1.2	13.1 ± 2.8	10.5 ± 1.2
	Necrosis	3.3 ± 0.6	3.2 ± 0.8	3.6 ± 0.3
12 μM	Healthy cells	88.7 ± 1.3	84.1 ± 2.8	82.9 ± 2.9
	Early apoptosis	2.3 ± 0.8	1.9 ± 0.3	3.9 ± 0.7
	Late apoptosis	5.5 ± 0.6	11.5 ± 2.7	9.9 ± 1.9
	Necrosis	3.5 ± 0.6	2.5 ± 0.4	3.3 ± 0.6