Migration and Proliferation Effects of Thymoquinone-Loaded Nanostructured Lipid Carrier (TQ-NLC) and Thymoquinone (TQ) on In Vitro Wound Healing Models

Table 5

Percentage of healthy, apoptotic, and necrotic cells of 3T3-L1 treated with TQ-NLC as determined by flow cytometry.

		24 h	48 h	72 h
Control	Healthy cells	85.9 ± 1.6	88.5 ± 0.7	88.5 ± 0.7
	Early apoptosis	2.2 ± 0.3	1.0 ± 0.1	3.8 ± 0.3
	Late apoptosis	9.7 ± 1.4	7.1 ± 0.6	4.3 ± 0.4
	Necrosis	2.2 ± 0.5	3.4 ± 0.2	3.4 ± 0.9
NLC	Healthy cells	87.0 ± 0.4	87.1 ± 0.8	86.3 ± 0.4
	Early apoptosis	2.8 ± 0.8	2.7 ± 0.1	2.9 ± 0.2
	Late apoptosis	8.3 ± 0.4	8.5 ± 0.1	8.9 ± 0.2
	Necrosis	1.9 ± 0.1	1.7 ± 0.3	1.9 ± 0.2
3 μM	Healthy cells	88.5 ± 1.2	86.4 ± 1.3	91.2 ± 2.0
	Early apoptosis	2.1 ± 0.7	1.5 ± 0.2	3.5 ± 0.2
	Late apoptosis	7.5 ± 0.7	8.6 ± 1.4	2.2 ± 1.7
	Necrosis	1.9 ± 0.4	3.5 ± 0.8	3.1 ± 0.0
6 μM	Healthy cells	90.3 ± 0.4	92.4 ± 0.5	89.4 ± 0.9
	Early apoptosis	2.4 ± 0.2	0.9 ± 0.2	4.4 ± 1.3
	Late apoptosis	6.2 ± 0.5	3.7 ± 0.5	2.6 ± 0.4
	Necrosis	1.1 ± 0.0	3.0 ± 0.2	3.6 ± 0.4
12 μM	Healthy cells	91.2 ± 0.2	92.6 ± 0.3	91.3 ± 2.1
	Early apoptosis	2.3 ± 0.4	0.7 ± 0.2	4.2 ± 1.4
	Late apoptosis	5.3 ± 1.1	2.5 ± 0.7	1.9 ± 1.4
	Necrosis	1.2 ± 0.7	4.2 ± 0.6	2.6 ± 0.5