Modulation of Diacylglycerol-Induced Melanogenesis in Human Melanoma and Primary Melanocytes: Role of Stress Chaperone Mortalin

Figure 2

Effect of OAG and TXC on mortalin, HSP70, and HSP60 expressions in human melanoma (G361) and primary melanocytes from Caucasian skin (PMC) as determined by immunocytochemistry using specific antibodies (a). Cells were treated with OAG for 24 h followed by recovery either in control (OAG) or TXC-supplemented medium as indicated. Effect of OAG and TXC on Reactive Oxygen Species (ROS) (b) and mitochondrial membrane potential (c) in human melanoma (G361) and primary melanocytes from Caucasian skin (PMC) as determined by live cell ROS detection and JCI staining, respectively. Cells were treated with OAG for 24 h followed by recovery either in the control (OAG) or TXC-supplemented medium as indicated.

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