Modulation of Diacylglycerol-Induced Melanogenesis in Human Melanoma and Primary Melanocytes: Role of Stress Chaperone Mortalin

Figure 3

Effect of selected natural extracts on OAG-induced increase in melanosomes (a) and melanin content (b) in human melanoma (G361) and primary melanocytes from Caucasian skin (PMC). Cells were treated with OAG for 24 h followed by recovery either in control (OAG) or extract (EX-33, EX-39, EX-45, and EX-46) supplemented medium as indicated. Increase in OAG-induced ROS and its reversal in the presence of extracts are shown (c). Increase in melanosomes, Tyrosinase, HSP60, and mortalin in OAG-treated human melanoma (G361) (d) and primary melanocytes from Caucasian skin (PMC) (e) and their reversal in response to treatment with extract (EX-33, EX-39, EX-45, and EX-46) are shown. Quantitation of the data obtained from three independent experiments is shown with each panel.