N-Butanol Extract of Gastrodia elata Suppresses Inflammatory Responses in Lipopolysaccharide-Stimulated Macrophages and Complete Freund’s Adjuvant- (CFA-) Induced Arthritis Rats via Inhibition of MAPK Signaling Pathway

Figure 5

The effect of BGE on NO secretion and inflammatory protein expression. (a) RAW264.7 mouse macrophage was treated with BGE (10, 20, and 40 μg/mL and LPS (1 μg/mL)) for 24 h, and LPS-induced NO production levels were determined by the NO assay kit. Data represent the mean ± SD of three independent experiments. (b) Immunoblot analysis of expression of iNOS and COX-2 in RAW264.7 mouse macrophage treated with BGE (10, 20, and 40 μg/mL)and LPS (1 μg/mL) for 24 h. (c) Quantitative analysis of gray value of iNOS and COX-2 was performed in several groups with β-actin as loading control. Data represent the mean ± SD of three independent experiments. , compared with the normal group. , , compared with the LPS group.

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