Research Article

Scrodentoids H and I, a Pair of Natural Epimerides from Scrophularia dentata, Inhibit Inflammation through JNK-STAT3 Axis in THP-1 Cells

Figure 2

Scrodentoids H and I pretreatment reduced the secretion of proinflammatory cytokines and mRNA of IL-1β and IL-6 in LPS-induced THP-1 macrophages. (a, b) Cells were pretreated with various concentrations of scrodentoids H and I (0∼60 μM) for 1 h following treatment of LPS (1 μg/mL) for 4 h, and total RNA was extracted to detect relative mRNA levels. (c, d) Treatment with scrodentoids H and I for 1 h and then LPS stimulated 24 hours to collect cell supernatants for detection of various protein expressions. All data are mean ± SEM, n = 3, vs. LPS-stimulated cells and vs. control.