Study on the Mechanism of Sarsasapogenin in Treating Precocious Puberty by Regulating the HPG Axis

Table 1

Primers’ sequence.

Name of the gene	Primer sequence (5′ to 3′)	Amplification fraction (bp)
GnRH	Forward: GGAGCTCTGGAACGTCTGATT; Reverse: CAGCGTCAATGTCACACTCG	100
GnRH-R	Forward: CAGGACCCACGCAAACTACA; Reverse: GGGAGTCCAGCAGATGACAA	117
Kiss-1	Forward: GCTGCTGCTTCTCCTCTGTGT; Reverse: CTGTTGGCCTGTGGGTTCA	88
GPR54	Forward: GCGGCCACAGATGTCACTTT; Reverse: AGGTGGGCAGCGGATAGAG	70
β-Actin	Forward: TGACAGGATGCAGAAGGAGA; Reverse: TAGAGCCACCAATCCACACA	104