Evidence-Based Complementary and Alternative Medicine / 2020 / Article / Fig 2

Research Article

Isoimperatorin Induces Apoptosis of Nasopharyngeal Carcinoma Cells via the MAPK/ERK1/2 Signaling Pathway

Figure 2

(a) Dual-fluorescence flow cytometry cellometer image cytometer (K2) was used to detect the change of apoptotic rate of CNE2 cells after isoimperatorin intervention. The apoptotic rate in the 10 μM group was 23.06 ± 2.00%, the 20 μM group was 22.90 ± 3.47%, and the 40 μM group was 29.06 ± 1.25% vs control group: . Cytation™ 5 cell imaging multifunctional detection system detects changes in the nucleus (b) and cell membrane potentials (c) of the cells after the intervention of isoimperatorin. (A) Control; (B)ISOIMP 10 μM; (C) ISOIMP20 μM; (D)ISOIMP 40 μM; (E) CIS 4.0 μg·mL–1(bar=100 μm, 200x). The nucleus appears bright blue with nuclear condensation. The increase in green fluorescence indicates a decrease in CNE2 cell membrane potentials and cell apoptosis. Isoimperatorin inhibits the expression of proliferation-related and apoptosis-related proteins in CNE2 cells. Western blot analysis of the effects of isoimperatorin at different concentrations on the relative expression of PCNA, XIAP, and survivin (d) and Bax and Bcl-2 (e) in CNE2 cells vs control group: ; .
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