Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy

Figure 4

Effects of bud and EEL on Bcl-2 and Bax. The protein area (a) and IOD (b) of Bcl-2 and Bax were measured by immunofluorescence staining. (c) Summarized data of mean density of Bcl-2 and Bax protein expression in lung tissues measured by WB, normalized to β-actin. (d) The mRNA expressions of Bcl-2 and Bax were measured by qPCR analysis with β-actin as an internal control. (e) Representative WB photographs of Bcl-2 and Bax were viewed, and β-actin was used for normalization. (f) The ratio of Bcl-2 to Bax was calculated in the level of relative protein measured by WB. (g) Representative immunofluorescence images of Bcl-2 and Bax (×200). Data are represented as mean ± SD; n = 6. and compared with control group; and compared with asthma group; and compared with bud group; compared with EEL group.

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