Combined Extracts of Epimedii Folium and Ligustri Lucidi Fructus with Budesonide Attenuate Airway Remodeling in the Asthmatic Rats by Regulating Apoptosis and Autophagy

Figure 6

Effects of Bud and EEL on LC3 and Beclin-1. The protein area (a) and IOD (b) of Beclin-1 were measured by immunofluorescence staining. (c) The mRNA expressions of Beclin-1 were measured by qPCR analysis with β-actin as an internal control. (d) Representative WB photographs of LC3 and Beclin-1 were viewed, and GAPDH was used for normalization. Summarized data of mean density of protein ratio of LC3-II/LC3-I (e) and Beclin-1 protein (f) in lung tissues measured by WB, normalized to GAPDH. (g) Representative immunofluorescence images of Beclin-1 (×200). Data are represented as mean ± SD; n = 6. compared with control group; compared with asthma group; compared with Bud group; compared with EEL group.

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