Anticancer Activity of Modified Tongyou Decoction on Eca109 Esophageal Cancer Cell Invasion and Metastasis through Regulation of the Epithelial-Mesenchymal Transition Mediated by the HIF-1α-Snail Axis

Figure 5

MTD inhibited Snail mRNA expression in both normoxia and hypoxia. Note: (a) amplification curve, as the target gene, Snail was measured with GAPDH. Value of Ct was in the domain of 15-30, and ▵Rn was in the domain of 0.5–1.5. (b) In Group OM, mRNA expression of Snail was inhibited remarkably, showing statistical difference from that in Group OC,  < 0.05. What is noteworthy, in Group HC, Snail mRNA overexpressed greatly, showing statistical difference from that in Group OC,  < 0.05. In Group HM, the mRNA expression was inhibited significantly, showing statistical difference from that in Group HC,  < 0.05. Homogeneity of variance by one-way ANOVA; LSD test applied to compare statistical difference.

(a)

(b)